Mycobacterium phage AlanGrant
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| Detailed Information for Phage AlanGrant | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Bumbpo |
| Found By | Margaret Farrell |
| Year Found | 2014 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.441 N, 79.95 W Map |
| Discovery Notes | Phage was not isolated from unenriched sample; first plaques were plated following the first enrichment procedure. |
| Naming Notes | Dr. Alan Grant is the paleontologist from the novel and movie Jurassic Park. He is a dynamic character, well-read, innovative, and quick-thinking, and also kicks a raptor in the face. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 17, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1147 |
| Genome length (bp) | 72109 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | AlanGrant forms a range of plaque sizes that are consistent through multiple rounds of purification. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR080200 |
| Refseq Number | None yet |
| Published in Paper | Pope WH et al 2015, Genome Announcements: Genome sequence of Mycobacteriophage Momo |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 1 |
| Pitt Freezer Box Grid# | C1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
