| Detailed Information for Phage Lolalove |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hannah Marie Rozier |
| Year Found | 2018 |
| Location Found | Charleston, SC USA |
| Finding Institution | College of Charleston |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.787021 N, 79.934143 E Map |
| Discovery Notes | Found at a Shell gas station. The soil was dark and wet.
Initially discovered and named by Hannah Marie Rozier. Additional work on sample was done by Cody Johnson and Stephanie Jackson. |
| Naming Notes | Named after my dog. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1193 |
| Genome length (bp) | 71111 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Sequencing Notes | In this sample, there was another Cluster B4 genome present. Relative coverages of discrepant contigs indicate that about 80% of the DNA belongs to the phage which we've posted as the official Lolalove, while 20% belongs to the second B4 phage. The lysate sample this DNA came from might be similarly mixed. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | College of Charleston |
| Annotation Status | Being Annotated (Expected completion by 5/15/2020) |
| Plaque Notes | TEM measurements from Hannah Marie Rozier:
Head Diameter = 87.5 nm
Tail Length = 365.6 nm
Tail Diameter = 12.5 nm
TEM measurements from Stephanie Jackson:
Head Diameter = 75 nm
Tail Length = 337.5 nm
Tail Diameter = 16.7 nm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |