Mycobacterium phage Lolalove
Lolalove Plaque Picture
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Detailed Information for Phage Lolalove
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHannah Marie Rozier
Year Found2018
Location FoundCharleston, SC USA
Finding InstitutionCollege of Charleston
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.787021 N, 79.934143 W Map
Discovery NotesFound at a Shell gas station. The soil was dark and wet.
Initially discovered and named by Hannah Marie Rozier. Additional work on sample was done by Cody Johnson and Stephanie Jackson.
Naming NotesNamed after my dog.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1193
Genome length (bp)71111
Character of genome endsCircularly Permuted
GC Content68.9%
Sequencing NotesIn this sample, there was another Cluster B4 genome present. Relative coverages of discrepant contigs indicate that about 80% of the DNA belongs to the phage which we've posted as the official Lolalove, while 20% belongs to the second B4 phage. The lysate sample this DNA came from might be similarly mixed.
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB4
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionCollege of Charleston
Annotation StatusIn GenBank
Plaque NotesTEM measurements by Christine Byrum (all photos collected for TEM from Jackson, Rozier, Johnson):
Capsid Diameter = 71.43 - 78.57nm, Avg = 76.83nm
Tail Length = 310 - 356.92 nm, Avg = 329.34nm
Tail Diameter = 10.94 - 12.86nm, Avg = 12.8nm

TEM measurements from Hannah Marie Rozier:
Head Diameter = 87.5 nm
Tail Length = 365.6 nm
Tail Diameter = 12.5 nm

TEM measurements from Stephanie Jackson:
Head Diameter = 75 nm
Tail Length = 337.5 nm
Tail Diameter = 16.7 nm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Lolalove_1
(ParB-like nuclease domain protein)

Lolalove_2
(hydroxylase)

Lolalove_3
(nucleotide pyrophosphohydrolase)

Lolalove_4
(adenylate kinase)

Lolalove_5
(terminase)

Lolalove_6

Lolalove_7
(RuvC-like resolvase)

Lolalove_8

Lolalove_9
(portal protein)

Lolalove_10
(capsid morphogenesis protein)

Lolalove_11

Lolalove_12
(major capsid protein)

Lolalove_13

Lolalove_14

Lolalove_15

Lolalove_16

Lolalove_17

Lolalove_18
(major tail protein)

Lolalove_19

Lolalove_20
(head-to-tail adaptor)

Lolalove_21

Lolalove_22

Lolalove_23
(tail assembly chaperone)

Lolalove_24

Lolalove_25
(tape measure protein)

Lolalove_26
(minor tail protein)

Lolalove_27
(minor tail protein)

Lolalove_28
(minor tail protein)

Lolalove_29
(minor tail protein)

Lolalove_30
(minor tail protein)

Lolalove_31

Lolalove_32

Lolalove_33

Lolalove_34

Lolalove_35

Lolalove_36

Lolalove_37

Lolalove_38

Lolalove_39

Lolalove_40
(helix-turn-helix DNA binding domain protein)

Lolalove_41
(helix-turn-helix DNA binding domain protein)

Lolalove_42

Lolalove_43

Lolalove_44
(lysin A)

Lolalove_45
(lysin B)

Lolalove_46

Lolalove_47
(exonuclease)

Lolalove_48
(DNA helicase)

Lolalove_49

Lolalove_50

Lolalove_51

Lolalove_52
(DNA primase/polymerase)

Lolalove_53

Lolalove_54
(DNA polymerase I)

Lolalove_55

Lolalove_56

Lolalove_57

Lolalove_58

Lolalove_59

Lolalove_60

Lolalove_61

Lolalove_62
(ribbon-helix-helix DNA binding domain protein)

Lolalove_63

Lolalove_64

Lolalove_65

Lolalove_66

Lolalove_67

Lolalove_68

Lolalove_69
(helix-turn-helix DNA binding domain protein)

Lolalove_70

Lolalove_71

Lolalove_72

Lolalove_73
(HNH endonuclease)

Lolalove_74

Lolalove_75

Lolalove_76

Lolalove_77
(HNH endonuclease)

Lolalove_78

Lolalove_79

Lolalove_80

Lolalove_81

Lolalove_82

Lolalove_83

Lolalove_84

Lolalove_85

Lolalove_86
(HicA-like toxin)

Lolalove_87

Lolalove_88

Lolalove_89

Lolalove_90

Lolalove_91

Lolalove_92

Lolalove_93

Lolalove_94

Lolalove_95

Lolalove_96

Lolalove_97

Lolalove_98

Lolalove_99

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT818419
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 93
Pitt Freezer Box Grid# E3
Available Files
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload