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Mycobacterium phage Allegro
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Detailed Information for Phage Allegro
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesCourtWalk
Found ByAshley Wood, Courtney Miller,Gabby W, Mason Linker
Year Found2020
Location FoundRock Hill, SC United States
Finding InstitutionWinthrop University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates34.9375 N, 81.033889 W Map
Discovery NotesThis phage was adopted from a classmate's enriched sample. The soil sample was collected one day after it rained. The outside temperature was cool, and the sample was collected about 2 cm deep.
Naming NotesAllegro is a music term that means fast. This phage is very aggressive. Also, with this phage, we have worked very fast and efficient. This also fits with the name Allegro.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1957
Genome length (bp)67439
Character of genome endsCircularly Permuted
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Lysogeny NotesThe phage is lytic.
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Dati (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

Prince (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Annotating InstitutionWinthrop University
Annotation StatusIn GenBank
Plaque NotesThe plaque was tiny, about the size of a pen tip, and was clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Allegro_1
(helix-turn-helix DNA binding domain protein)

Allegro_2
(queuine tRNA-ribosyltransferase)

Allegro_3
(Pre Qo pathway QueC-like protein)

Allegro_4
(Pre Qo pathway QueD-like protein)

Allegro_5
(Pre Qo pathway QueE-like protein)

Allegro_6
(GTP cyclohydrolase I)

Allegro_7
(terminase)

Allegro_8

Allegro_9
(RuvC-like resolvase)

Allegro_10

Allegro_11

Allegro_12
(portal protein)

Allegro_13
(MuF-like minor capsid protein)

Allegro_14

Allegro_15
(major capsid protein)

Allegro_16

Allegro_17

Allegro_18

Allegro_19

Allegro_20

Allegro_21
(major tail protein)

Allegro_22

Allegro_23

Allegro_24
(head-to-tail adaptor)

Allegro_25

Allegro_26

Allegro_27
(tail assembly chaperone)

Allegro_28
(tail assembly chaperone)

Allegro_29
(tape measure protein)

Allegro_30
(minor tail protein)

Allegro_31
(minor tail protein)

Allegro_32
(minor tail protein)

Allegro_33
(minor tail protein)

Allegro_34

Allegro_35

Allegro_36

Allegro_37

Allegro_38

Allegro_39

Allegro_40

Allegro_41

Allegro_42

Allegro_43

Allegro_44
(helix-turn-helix DNA binding domain protein)

Allegro_45
(helix-turn-helix DNA binding domain protein)

Allegro_46

Allegro_47
(lysin A)

Allegro_48
(holin)

Allegro_49

Allegro_50

Allegro_51
(DNA helicase)

Allegro_52

Allegro_53

Allegro_54

Allegro_55
(DNA primase/helicase)

Allegro_56

Allegro_57
(DNA polymerase I)

Allegro_58

Allegro_59

Allegro_60

Allegro_61

Allegro_62

Allegro_63

Allegro_64
(ribbon-helix-helix DNA binding domain protein)

Allegro_65

Allegro_66
(DNA binding protein)

Allegro_67

Allegro_68

Allegro_69
(DNA binding protein)

Allegro_70
(HNH endonuclease)

Allegro_71

Allegro_72

Allegro_73

Allegro_74

Allegro_75

Allegro_76

Allegro_77

Allegro_78

Allegro_79

Allegro_80

Allegro_81

Allegro_82

Allegro_83

Allegro_84

Allegro_85

Allegro_86

Allegro_87

Allegro_88

Allegro_89

Allegro_90

Allegro_91

Allegro_92

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ456036
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.67 x 10^9 pfu/mL
Pitt Freezer Box# 118
Pitt Freezer Box Grid# F11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload