Mycobacterium phage Surely
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Detailed Information for Phage Surely
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEmma and Annie
Year Found2015
Location FoundKenosha, WI USA
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.62476 N, 87.822168 W Map
Discovery NotesSurely was discovered in Kenosha, WI on Carthage College Campus near the shore of Lake Michigan. It was found in moist soil located under a bridge.
Naming NotesSurely was named because it was a slow and steady process to both enrich and cultivate her. Slowly but surely our phage was discovered.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 19, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage412
Genome length (bp)68888
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Boehler (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionCarthage College
Annotation StatusIn GenBank
Plaque NotesSurely was temperate with clear centers and large cloudy halos.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Surely_1
(adenylate kinase)

Surely_2
(terminase)

Surely_3

Surely_4

Surely_5

Surely_6
(RuvC-like resolvase)

Surely_7
(membrane protein)

Surely_8
(portal protein)

Surely_9
(capsid maturation protease and MuF-like fusion protein)

Surely_10
(HNH endonuclease)

Surely_11

Surely_12
(major capsid protein)

Surely_13

Surely_14

Surely_15
(membrane protein)

Surely_16
(membrane protein)

Surely_17
(membrane protein)

Surely_18
(major tail protein)

Surely_19

Surely_20
(queuine tRNA-ribosyltransferase)

Surely_21

Surely_22
(head-to-tail adaptor)

Surely_23

Surely_24

Surely_25
(tail assembly chaperone)

Surely_26

Surely_27

Surely_28
(tape measure protein)

Surely_29
(minor tail protein)

Surely_30
(minor tail protein)

Surely_31
(minor tail protein)

Surely_32
(minor tail protein)

Surely_33
(minor tail protein)

Surely_34

Surely_35

Surely_36

Surely_37

Surely_38

Surely_39

Surely_40

Surely_41

Surely_42

Surely_43

Surely_44

Surely_45
(helix-turn-helix DNA binding domain protein)

Surely_46
(helix-turn-helix DNA binding domain protein)

Surely_47

Surely_48
(lysin A)

Surely_49
(lysin B)

Surely_50

Surely_51

Surely_52
(DNA helicase)

Surely_53

Surely_54

Surely_55

Surely_56

Surely_57

Surely_58
(DNA primase/polymerase)

Surely_59

Surely_60
(DNA polymerase I)

Surely_61

Surely_62

Surely_63

Surely_64

Surely_65

Surely_66

Surely_67

Surely_68

Surely_69

Surely_70
(helix-turn-helix DNA binding protein)

Surely_71

Surely_72

Surely_73

Surely_74

Surely_75
(HNH endonuclease)

Surely_76

Surely_77

Surely_78

Surely_79

Surely_80

Surely_81

Surely_82

Surely_83

Surely_84
(HNH endonuclease)

Surely_85

Surely_86

Surely_87

Surely_88

Surely_89

Surely_90

Surely_91

Surely_92

Surely_93

Surely_94

Surely_95

Surely_96

Surely_97

Surely_98
(membrane protein)

Surely_99

Surely_100

Surely_101

Surely_102

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279885
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.4 x 10^10
Pitt Freezer Box# 28
Pitt Freezer Box Grid# H4
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload