Mycobacterium phage Chunky
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Detailed Information for Phage Chunky
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJoanna Smith & Brian Lott
Year Found2013
Location FoundSanta Cruz, CA USA
Finding InstitutionUniversity of California, Santa Cruz
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.991525 N, 122.058426 W Map
Discovery NotesFound in a compost pile at the UCSC Center for Agroecology, about 1 inch deep in the compost pile. Phage discovered, purified, and named by Joanna Smith. DNA purification and restriction digests were performed by Brian Lott.
Naming NotesTwo separate phages were isolated from the same sample. During purification their morphologies were noticeably different, one was small (called 'Teenie') and the other was very large (appropriately named 'Chunky'.)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1941
Genome length (bp)68249
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Boehler (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionUniversity of California, Santa Cruz
Annotation StatusIn GenBank
Plaque NotesChunky forms large, clear plaques that range in size from 3mm to 5mm. Average plaque size would be ~3.5mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Chunky_1
(adenylate kinase)

Chunky_2
(terminase)

Chunky_3

Chunky_4

Chunky_5

Chunky_6
(RuvC-like resolvase)

Chunky_7

Chunky_8
(portal protein)

Chunky_9
(capsid maturation protease and MuF-like fusion protein)

Chunky_10
(HNH endonuclease)

Chunky_11

Chunky_12
(major capsid protein)

Chunky_13

Chunky_14

Chunky_15

Chunky_16

Chunky_17

Chunky_18
(major tail protein)

Chunky_19

Chunky_20
(queuine tRNA-ribosyltransferase)

Chunky_21

Chunky_22
(head-to-tail adaptor)

Chunky_23

Chunky_24

Chunky_25
(tail assembly chaperone)

Chunky_26

Chunky_27

Chunky_28
(tape measure protein)

Chunky_29
(minor tail protein)

Chunky_30
(minor tail protein)

Chunky_31
(minor tail protein)

Chunky_32
(minor tail protein)

Chunky_33
(minor tail protein)

Chunky_34

Chunky_35

Chunky_36

Chunky_37

Chunky_38

Chunky_39

Chunky_40

Chunky_41

Chunky_42

Chunky_43

Chunky_44

Chunky_45
(helix-turn-helix DNA binding domain protein)

Chunky_46
(MeR-like HTH DNA binding protein)

Chunky_47

Chunky_48
(lysin A)

Chunky_49
(lysin B)

Chunky_50

Chunky_51

Chunky_52
(DNA helicase)

Chunky_53

Chunky_54

Chunky_55

Chunky_56
(DNA primase/polymerase)

Chunky_57

Chunky_58

Chunky_59
(DNA polymerase I)

Chunky_60

Chunky_61

Chunky_62

Chunky_63

Chunky_64

Chunky_65

Chunky_66

Chunky_67

Chunky_68

Chunky_69

Chunky_70
(RNAseE)

Chunky_71

Chunky_72

Chunky_73

Chunky_74

Chunky_75
(HTH DNA binding domain protein)

Chunky_76

Chunky_77

Chunky_78

Chunky_79

Chunky_80

Chunky_81

Chunky_82

Chunky_83

Chunky_84
(HNH endonuclease)

Chunky_85

Chunky_86

Chunky_87

Chunky_88

Chunky_89

Chunky_90

Chunky_91

Chunky_92

Chunky_93

Chunky_94

Chunky_95

Chunky_96

Chunky_97

Chunky_98

Chunky_99

Chunky_100

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG925339
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.5x10^9 pfu/mL
Date of SEA Lysate Titering Nov 27, 2013
Pitt Freezer Box# 43
Pitt Freezer Box Grid# 32
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload