Mycobacterium phage Inchworm
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Detailed Information for Phage Inchworm
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCecilia Petruconis
Year Found2019
Location FoundPhiladelphia, PA United States
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature36°C
GPS Coordinates39.9941 N, 75.2379 W Map
Discovery NotesInchworm was found under the large pine tree at the top of the staircase next to Bellarmine Hall on the Saint Joseph's University Campus. Inchworm came from Direct Isolation, followed by 2 more rounds of purification. The Titer was 3.8 x 10^10 pfu/mL. The DNA concentration was 111.6 μg/mL. The average capsid size was 58.3 nm in diameter, and the average tail was 312.5 nm in length.
Naming NotesWhen I collected this sample, I dug up an inchworm and put it into the sample collection by accident. I removed the inchworm before returning to the lab, but the name stuck.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2020
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2173
Genome length (bp)68929
Character of genome endsCircularly Permuted
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionSaint Joseph's University
Annotation StatusIn GenBank
Plaque NotesThe plaques for all rounds of purification were 3 mm diameter. They were clear plaques, reflecting a lytic phage. They were symmetrical and had clear edges and were consistent throughout isolation.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Inchworm_1
(adenylate kinase)

Inchworm_2
(terminase)

Inchworm_3

Inchworm_4

Inchworm_5

Inchworm_6
(RuvC-like resolvase)

Inchworm_7

Inchworm_8
(portal protein)

Inchworm_9
(capsid maturation protease and MuF like fusion)

Inchworm_10
(HNH endonuclease)

Inchworm_11

Inchworm_12
(major capsid protein)

Inchworm_13

Inchworm_14

Inchworm_15

Inchworm_16

Inchworm_17

Inchworm_18
(major tail protein)

Inchworm_19

Inchworm_20
(dpdA-like tRNA-guanine transglycosylase)

Inchworm_21

Inchworm_22
(head-to-tail adaptor)

Inchworm_23

Inchworm_24

Inchworm_25
(tail assembly chaperone)

Inchworm_26

Inchworm_27

Inchworm_28
(tape measure protein)

Inchworm_29
(minor tail protein)

Inchworm_30
(minor tail protein)

Inchworm_31
(minor tail protein)

Inchworm_32
(minor tail protein)

Inchworm_33
(minor tail protein)

Inchworm_34

Inchworm_35

Inchworm_36

Inchworm_37

Inchworm_38

Inchworm_39

Inchworm_40

Inchworm_41

Inchworm_42

Inchworm_43

Inchworm_44

Inchworm_45
(helix-turn-helix DNA binding domain)

Inchworm_46
(helix-turn-helix DNA binding domain)

Inchworm_47

Inchworm_48

Inchworm_49
(lysin A)

Inchworm_50
(lysin B)

Inchworm_51

Inchworm_52

Inchworm_53
(DNA helicase)

Inchworm_54

Inchworm_55

Inchworm_56

Inchworm_57

Inchworm_58

Inchworm_59

Inchworm_60
(DNA primase/polymerase)

Inchworm_61

Inchworm_62
(DNA polymerase I)

Inchworm_63

Inchworm_64

Inchworm_65

Inchworm_66

Inchworm_67

Inchworm_68

Inchworm_69

Inchworm_70

Inchworm_71
(helix-turn-helix DNA binding domain)

Inchworm_72

Inchworm_73

Inchworm_74

Inchworm_75

Inchworm_76
(HNH endonuclease)

Inchworm_77

Inchworm_78

Inchworm_79

Inchworm_80

Inchworm_81

Inchworm_82

Inchworm_83

Inchworm_84

Inchworm_85
(HNH endonuclease)

Inchworm_86

Inchworm_87

Inchworm_88

Inchworm_89

Inchworm_90

Inchworm_91

Inchworm_92

Inchworm_93

Inchworm_94

Inchworm_95

Inchworm_96

Inchworm_97

Inchworm_98

Inchworm_99

Inchworm_100

Inchworm_101

Inchworm_102

Inchworm_103

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT889369
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.1 x 10^9 pfu/mL
Pitt Freezer Box# 101
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload