Mycobacterium phage Altwerkus
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Detailed Information for Phage Altwerkus
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAugusto Ramirez
Year Found2013
Location FoundBaltimore, MD United States
Finding InstitutionJohns Hopkins University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates39.331256 N, 76.620347 W Map
Discovery NotesSoil Sample: (09/03/13 at 8:44 PM)
-Found in the John Hopkins University Bufano Gardens (North of the Undergraduate Teaching Lab)
-Air Temperature: 25°C
-Depth: 1.5 cm
-Moisture Content: Dry
-Soil was close to a tree and fallen leaves
-Insects crawling in the area

Phage Characteristics:
-Head Length: ~88 nm
-Head Width: ~83 nm
-Tail Length: ~418 nm
Naming NotesAl(twerk)us. From the latin word altus, for tall, because of the great length of this phage's tail and the rising trend of this current generation called "twerking," which involves the movement of the tail.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 3, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage547
Genome length (bp)68179
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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Alis (None)

Azarath (None)

BirdsNest (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Indlovu (None)

Kerrigan (None)

LilMcDreamy (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Benjamin1706 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

Emiris (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Giraffey (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Thonko (B8)

Annotation StatusIn GenBank
Plaque Notes-Plaque Diameter: 2 mm - 3 mm
-Inside: Clear Plaques
-Shape: Very Circular and Rounded
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Altwerkus_1
(adenylate kinase)

Altwerkus_2
(terminase)

Altwerkus_3

Altwerkus_4

Altwerkus_5

Altwerkus_6
(RuvC-like resolvase)

Altwerkus_7

Altwerkus_8
(portal protein)

Altwerkus_9
(capsid maturation protease and MuF-like fusion protein)

Altwerkus_10

Altwerkus_11
(major capsid protein)

Altwerkus_12

Altwerkus_13

Altwerkus_14
(membrane protein)

Altwerkus_15
(membrane protein)

Altwerkus_16
(membrane protein)

Altwerkus_17
(major tail)

Altwerkus_18

Altwerkus_19
(queuine tRNA-ribosyltransferase)

Altwerkus_20

Altwerkus_21
(head-to-tail adaptor)

Altwerkus_22

Altwerkus_23

Altwerkus_24
(tail assembly chaperone)

Altwerkus_25

Altwerkus_26

Altwerkus_27
(tape measure protein)

Altwerkus_28
(minor tail protein)

Altwerkus_29
(minor tail protein)

Altwerkus_30
(minor tail protein)

Altwerkus_31
(minor tail protein)

Altwerkus_32
(minor tail protein)

Altwerkus_33

Altwerkus_34

Altwerkus_35

Altwerkus_36

Altwerkus_37

Altwerkus_38

Altwerkus_39

Altwerkus_40

Altwerkus_41

Altwerkus_42

Altwerkus_43

Altwerkus_44
(helix-turn-helix DNA binding domain)

Altwerkus_45
(helix-turn-helix DNA binding domain)

Altwerkus_46

Altwerkus_47
(lysin A)

Altwerkus_48
(lysin B)

Altwerkus_49

Altwerkus_50

Altwerkus_51
(DNA helicase)

Altwerkus_52

Altwerkus_53

Altwerkus_54

Altwerkus_55

Altwerkus_56
(DNA primase/polymerase)

Altwerkus_57

Altwerkus_58
(DNA polymerase I)

Altwerkus_59

Altwerkus_60

Altwerkus_61

Altwerkus_62

Altwerkus_63

Altwerkus_64

Altwerkus_65

Altwerkus_66

Altwerkus_67

Altwerkus_68

Altwerkus_69
(helix-turn-helix DNA binding protein)

Altwerkus_70

Altwerkus_71

Altwerkus_72

Altwerkus_73

Altwerkus_74
(HNH endonuclease)

Altwerkus_75

Altwerkus_76

Altwerkus_77

Altwerkus_78

Altwerkus_79

Altwerkus_80

Altwerkus_81

Altwerkus_82
(HNH endonuclease)

Altwerkus_83

Altwerkus_84

Altwerkus_85

Altwerkus_86

Altwerkus_87

Altwerkus_88

Altwerkus_89

Altwerkus_90

Altwerkus_91

Altwerkus_92

Altwerkus_93

Altwerkus_94

Altwerkus_95
(membrane protein)

Altwerkus_96

Altwerkus_97

Altwerkus_98

Altwerkus_99

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK112527
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 13
Pitt Freezer Box Grid# D3
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload