Mycobacterium phage Hamish
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Detailed Information for Phage Hamish
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesNicolasPhage, Bellamy
Found ByHallie Smith
Year Found2016
Location FoundRock Hill, SC USA
Finding InstitutionWinthrop University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates34.9371 N, 81.032 W Map
Discovery NotesDry soil approximately 1 inch under top layer of soil. Obtained at 10:30 pm on 9/7/16 at Hardin Family Garden at Winthrop University. 29.44 °C
Naming NotesNamed after John Hamish Watson from BBC Sherlock.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 23, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage824
Genome length (bp)68584
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Dati (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

Austelle (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

Prince (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Annotating InstitutionWinthrop University
Annotation StatusIn GenBank
Plaque NotesAverage head diameter: 70.382 nm
Average tail length: 345.908 nm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Hamish_1
(adenylate kinase)

Hamish_2
(terminase)

Hamish_3

Hamish_4

Hamish_5

Hamish_6
(RuvC-like resolvase)

Hamish_7

Hamish_8
(portal protein)

Hamish_9
(capsid maturation protease and MuF-like fusion protein)

Hamish_10
(HNH endonuclease)

Hamish_11

Hamish_12
(major capsid hexamer protein)

Hamish_13
(major capsid pentamer protein)

Hamish_14

Hamish_15

Hamish_16

Hamish_17

Hamish_18
(major tail protein)

Hamish_19

Hamish_20
(queuine tRNA-ribosyltransferase)

Hamish_21

Hamish_22
(head-to-tail adaptor)

Hamish_23

Hamish_24

Hamish_25
(tail assembly chaperone)

Hamish_26

Hamish_27

Hamish_28
(tape measure protein)

Hamish_29
(minor tail protein)

Hamish_30
(minor tail protein)

Hamish_31
(minor tail protein)

Hamish_32
(minor tail protein)

Hamish_33
(minor tail protein)

Hamish_34

Hamish_35

Hamish_36

Hamish_37

Hamish_38

Hamish_39

Hamish_40

Hamish_41

Hamish_42

Hamish_43

Hamish_44

Hamish_45
(helix-turn-helix DNA binding domain protein)

Hamish_46
(helix-turn-helix DNA-binding protein)

Hamish_47

Hamish_48
(lysin A)

Hamish_49
(lysin B)

Hamish_50

Hamish_51

Hamish_52
(DNA helicase)

Hamish_53

Hamish_54

Hamish_55

Hamish_56

Hamish_57
(DNA primase/helicase)

Hamish_58

Hamish_59
(DNA polymerase I)

Hamish_60

Hamish_61

Hamish_62

Hamish_63

Hamish_64

Hamish_65

Hamish_66

Hamish_67

Hamish_68

Hamish_69

Hamish_70

Hamish_71

Hamish_72

Hamish_73

Hamish_74

Hamish_75

Hamish_76

Hamish_77

Hamish_78

Hamish_79

Hamish_80

Hamish_81
(HNH endonuclease)

Hamish_82

Hamish_83

Hamish_84

Hamish_85

Hamish_86

Hamish_87

Hamish_88

Hamish_89

Hamish_90

Hamish_91

Hamish_92

Hamish_93

Hamish_94

Hamish_95

Hamish_96

Hamish_97

Hamish_98

Hamish_99

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH825702
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.3e10 pfu/ml
Date of SEA Lysate Titering Sep 22, 2016
Pitt Freezer Box# 43
Pitt Freezer Box Grid# B3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload