Mycobacterium phage Mikota
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Detailed Information for Phage Mikota
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeams Lyse Hard & Orange is the New Plaque
Year Found2015
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.06 N, 118.45 W Map
Discovery NotesSample collected by Raul Alejandro Ramirez. Taken from under shrub leaf litter in soil patch next to the lower driveway of the UCLA university apartment Gayley Towers
-Depth from which sample was taken: 3.8 cm
-Air temperature: 27º C

Phage isolation, purification, characterization, and genome annotation conducted by Teams Lyse Hard & Orange is the New Plaque.

Lyse Hard:
Raul Alejandro Ramirez, Bowen Wang, Chris Wang

Orange is the New Plaque:
Emily Hsu, Justin Saco, Munawaer Ainiwaer, Nanor
Haladjian

TA: Rocky Ng, Instructor Giorgia Pirino
Naming NotesMikota; named after Bowen “Mike” Wang
who purified the phage, also a tribute to Rocky’s ‘Cota’ phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1007
Genome length (bp)67768
Character of genome endsCircularly Permuted
GC Content66.6%
Sequencing Notesmixed, 30% reads Blast 100% Cluster K1 Amelie
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Lysogeny NotesNo evidence for lysogeny due to clear plaque morphology and absence of integrase gene.
Other Cluster Members

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BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPrimarily clear

Average size (diameter in mm): 3.5
MorphotypeSiphoviridae
Number of Genes96
Has been Phamerated?Yes
Gene List

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Mikota_1
(adenylate kinase)

Mikota_2
(terminase)

Mikota_3

Mikota_4

Mikota_5

Mikota_6
(RuvC-like resolvase)

Mikota_7

Mikota_8
(portal protein)

Mikota_9
(capsid maturation protease and MuF-like fusion protein)

Mikota_10
(HNH endonuclease)

Mikota_11

Mikota_12
(major capsid protein)

Mikota_13

Mikota_14

Mikota_15

Mikota_16

Mikota_17

Mikota_18
(major tail protein)

Mikota_19

Mikota_20
(queuine tRNA-ribosyltransferase)

Mikota_21

Mikota_22
(head-to-tail adaptor)

Mikota_23

Mikota_24

Mikota_25
(tail assembly chaperone)

Mikota_26

Mikota_27

Mikota_28
(tape measure protein)

Mikota_29
(minor tail protein)

Mikota_30
(minor tail protein)

Mikota_31
(minor tail protein)

Mikota_32
(minor tail protein)

Mikota_33
(minor tail protein)

Mikota_34

Mikota_35

Mikota_36

Mikota_37

Mikota_38

Mikota_39

Mikota_40

Mikota_41

Mikota_42

Mikota_43

Mikota_44

Mikota_45
(helix-turn-helix DNA binding domain protein)

Mikota_46
(helix-turn-helix DNA binding domain protein)

Mikota_47

Mikota_48
(lysin A)

Mikota_49
(lysin B)

Mikota_50

Mikota_51

Mikota_52
(DNA helicase)

Mikota_53

Mikota_54

Mikota_55

Mikota_56
(DNA primase/polymerase)

Mikota_57

Mikota_58

Mikota_59
(DNA polymerase I)

Mikota_60

Mikota_61

Mikota_62

Mikota_63

Mikota_64

Mikota_65

Mikota_66

Mikota_67
(endoribonuclease)

Mikota_68

Mikota_69

Mikota_70

Mikota_71

Mikota_72
(helix-turn-helix DNA binding domain protein)

Mikota_73

Mikota_74

Mikota_75

Mikota_76

Mikota_77

Mikota_78

Mikota_79

Mikota_80

Mikota_81
(HNH endonuclease)

Mikota_82

Mikota_83

Mikota_84

Mikota_85

Mikota_86

Mikota_87

Mikota_88

Mikota_89

Mikota_90

Mikota_91

Mikota_92

Mikota_93

Mikota_94

Mikota_95

Mikota_96

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919523
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 23
Pitt Freezer Box Grid# E7
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload