Mycobacterium phage Thonko
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Detailed Information for Phage Thonko
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNomusa Christina Mzimela
Year Found2017
Location FoundEmpangeni, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates28.8 S, 31.8 E Map
Discovery NotesThonko phage was discovered by Nomusa Christina Mzimela at a city of Empangeni in a place called Kwadlangezwa. It is a reddish brown soil collected from Mzimela residence yard.It was enriched with 7H9 media and incubated for 24 hours.
Naming NotesThonko is a name discovered fro my parents yard since I took the soil from home.I used the 3 letters of their names Thokozani(father) and Nkosingiphile(mother)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 18, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1574
Genome length (bp)69471
Character of genome endsCircularly Permuted
GC Content68.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB8
Cluster Life CycleLytic
Other Cluster Members

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Alis (None)

Azarath (None)

BirdsNest (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Indlovu (None)

Kerrigan (None)

LilMcDreamy (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

Emiris (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Giraffey (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Thonko (B8)

Annotation StatusIn GenBank
Plaque Notes"Thonko" took a heart shape when growing then after 48 hours it grew and took a Y shape.It was transparent in colour. It has titre 5 x 10^11
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Thonko_1
(terminase)

Thonko_2

Thonko_3
(RuvC-like resolvase)

Thonko_4

Thonko_5

Thonko_6

Thonko_7
(portal protein)

Thonko_8
(capsid morphogenesis protein)

Thonko_9

Thonko_10

Thonko_11

Thonko_12

Thonko_13
(major capsid protein)

Thonko_14

Thonko_15

Thonko_16
(membrane protein)

Thonko_17
(membrane protein)

Thonko_18
(membrane protein)

Thonko_19
(major tail protein)

Thonko_20

Thonko_21
(head-to-tail connector complex protein)

Thonko_22

Thonko_23

Thonko_24
(tail assembly chaperone)

Thonko_25
(tail assembly chaperone)

Thonko_26
(tape measure protein)

Thonko_27
(minor tail protein)

Thonko_28
(minor tail protein)

Thonko_29
(minor tail protein)

Thonko_30
(minor tail protein)

Thonko_31

Thonko_32

Thonko_33

Thonko_34

Thonko_35

Thonko_36

Thonko_37

Thonko_38

Thonko_39

Thonko_40

Thonko_41

Thonko_42
(membrane protein)

Thonko_43
(helix-turn-helix DNA binding domain protein)

Thonko_44
(helix-turn-helix DNA binding domain protein)

Thonko_45

Thonko_46
(lysin A)

Thonko_47
(lysin B)

Thonko_48

Thonko_49

Thonko_50

Thonko_51

Thonko_52

Thonko_53

Thonko_54
(DNA helicase)

Thonko_55
(MazG-like nucleotide pyrophosphohydrolase)

Thonko_56

Thonko_57
(RepA-like helicase)

Thonko_58

Thonko_59

Thonko_60
(DNA polymerase I)

Thonko_61

Thonko_62

Thonko_63

Thonko_64

Thonko_65

Thonko_66

Thonko_67
(ribbon-helix-helix DNA binding domain protein)

Thonko_68

Thonko_69

Thonko_70

Thonko_71

Thonko_72

Thonko_73

Thonko_74

Thonko_75

Thonko_76

Thonko_77

Thonko_78

Thonko_79

Thonko_80
(HNH endonuclease)

Thonko_81
(NrdH-like glutaredoxin)

Thonko_82

Thonko_83

Thonko_84

Thonko_85

Thonko_86
(helix-turn-helix DNA binding domain protein)

Thonko_87
(HNH endonuclease)

Thonko_88

Thonko_89

Thonko_90

Thonko_91

Thonko_92
(HicA-like toxin)

Thonko_93

Thonko_94
(metallophosphoesterase)

Thonko_95

Thonko_96

Thonko_97

Thonko_98

Thonko_99

Thonko_100

Thonko_101

Thonko_102

Thonko_103

Thonko_104

Thonko_105

Thonko_106

Thonko_107

Thonko_108

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH632120
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload