Mycobacterium phage MiniBoss
MiniBoss Plaque Picture
MiniBoss EM Picture
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Detailed Information for Phage MiniBoss
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKayla Davis
Year Found2022
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.06279 N, 118.44229 W Map
Discovery NotesAL Quarter and Lab section: F22-1C
Instructor: Tejas Bouklas
Teaching Assistant: Dom Garza
Undergraduate Assistant(s): Yuri Lin

Team #1 Name: MIMG BOATERS
Team #1 Members: Kayla Davis, Truc Pham, Matthew Santilla

Team #2 Name: Phager Danger
Team #2 Members: Ven Chavez, Irene Ulitsky, Marbella Garduno, Callum Guo

Team #3 Name: 4UPhage Queens
Team #3 Members: Clarice Chui, Kyleigh Kimbrell, Veronica Garcia, Luke Kristianto

Team #4 Name: 11th Hour
Team #3 Members: Alex Ortiz, Min Jin Kim, Sophia Pulido, Jacqueline Quach
Naming NotesThis phage was named after Gru, the main character in the movie Minions: The Rise of Gru (2022 Animation Film).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 8, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1384
Genome length (bp)67386
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

Basato (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

BlueHusk (B1)

Bluephacebaby (B1)

Boehler (B1)

Brilliant (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

CampRoach (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Chute (B1)

Cobra (B1)

Colbert (B1)

Coletti (B1)

Cornobble (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Daka (B1)

Dati (B1)

DelRivs (B1)

Derpp (B1)

Dice (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Fozzie (B1)

Frankicide (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gwilliam (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Hartsy (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

JDog (B1)

Jillium (B1)

Jiminy (B1)

Jiraiya (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

LasagnaCat (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

Lopsy (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Lumine (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megamind20 (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MiniBoss (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

MrPhizzler (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Nyala (B1)

Olak (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orfeu (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

Pacifista (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhatLouie (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

Quisquiliae (B1)

RedMaple (B1)

Ricotta (B1)

Riggan (B1)

Rimu (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

Scrick (B1)

SDcharge11 (B1)

Selr12 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

SilverBell (B1)

Simielle (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swiphy (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Thunderbird (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Hashim76 (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kaleb (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Vic9 (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Ben11 (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Deenasa (B3)

Devonte (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

Halfpint (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Kronus (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phayeta (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SlippinJimmy (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

Ahwei (B4)

AlanGrant (B4)

Antihero (B4)

Apex (B4)

Austelle (B4)

BobbyK (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Epah (B4)

Fanaw (B4)

Fortunato (B4)

Frederick (B4)

GinPorsche (B4)

Hally898 (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JaguarMi (B4)

JAMaL (B4)

Lambano (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Mudslide (B4)

Nanao (B4)

Nigel (B4)

Poster (B4)

Prince (B4)

QueenPie (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Xincheng (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

DigitDog (B5)

Donny (B5)

EastView9101 (B5)

KarasumaRum (B5)

Mysterious (B5)

Nangang (B5)

Nehemiah (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Shenyu (B5)

SirJeffery (B5)

StinkyVeggy (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Rinkes (B9)

Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque Noteshalo, turbid, fuzzy margin
4mm diameter
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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MiniBoss_1
(adenylate kinase)

MiniBoss_2
(terminase)

MiniBoss_3

MiniBoss_4

MiniBoss_5

MiniBoss_6
(RuvC-like resolvase)

MiniBoss_7

MiniBoss_8
(portal protein)

MiniBoss_9
(capsid maturation protease)

MiniBoss_10
(HNH endonuclease)

MiniBoss_11

MiniBoss_12
(major capsid hexamer protein)

MiniBoss_13
(major capsid pentamer protein)

MiniBoss_14

MiniBoss_15

MiniBoss_16

MiniBoss_17

MiniBoss_18
(major tail protein)

MiniBoss_19

MiniBoss_20
(DpdA-like tRNA-guanine transglycosylase)

MiniBoss_21

MiniBoss_22
(head-to-tail adaptor)

MiniBoss_23

MiniBoss_24

MiniBoss_25

MiniBoss_26

MiniBoss_27

MiniBoss_28
(tape measure protein)

MiniBoss_29
(minor tail protein)

MiniBoss_30
(minor tail protein)

MiniBoss_31
(minor tail protein)

MiniBoss_32
(minor tail protein)

MiniBoss_33
(minor tail protein)

MiniBoss_34

MiniBoss_35

MiniBoss_36

MiniBoss_37

MiniBoss_38

MiniBoss_39

MiniBoss_40

MiniBoss_41

MiniBoss_42

MiniBoss_43

MiniBoss_44

MiniBoss_45
(helix-turn-helix DNA-binding domain protein)

MiniBoss_46
(helix-turn-helix DNA-binding domain protein)

MiniBoss_47

MiniBoss_48
(lysin A)

MiniBoss_49
(lysin B)

MiniBoss_50

MiniBoss_51
(exonuclease)

MiniBoss_52
(DNA helicase)

MiniBoss_53

MiniBoss_54

MiniBoss_55

MiniBoss_56
(DNA primase/polymerase/helicase)

MiniBoss_57

MiniBoss_58
(DNA polymerase I)

MiniBoss_59

MiniBoss_60

MiniBoss_61

MiniBoss_62

MiniBoss_63

MiniBoss_64

MiniBoss_65

MiniBoss_66

MiniBoss_67
(helix-turn-helix DNA-binding domain protein)

MiniBoss_68

MiniBoss_69

MiniBoss_70

MiniBoss_71

MiniBoss_72

MiniBoss_73

MiniBoss_74

MiniBoss_75

MiniBoss_76

MiniBoss_77

MiniBoss_78

MiniBoss_79

MiniBoss_80

MiniBoss_81
(HNH endonuclease)

MiniBoss_82

MiniBoss_83

MiniBoss_84

MiniBoss_85

MiniBoss_86

MiniBoss_87

MiniBoss_88

MiniBoss_89

MiniBoss_90

MiniBoss_91

MiniBoss_92

MiniBoss_93

MiniBoss_94

MiniBoss_95

MiniBoss_96

MiniBoss_97

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR434024
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 155
Pitt Freezer Box Grid# H6
Available Files
GenBank File for PhameratorDownload