Mycobacterium phage Squiggle
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Detailed Information for Phage Squiggle
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaleb Curry
Year Found2016
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.98112 N, 86.456531 W Map
Discovery NotesThis phage was found in the compost pile at the Western Kentucky University Office of Sustainability. The soil had a low/medium moisture content, and was full of organic matter. I collected the sample from the surface of the compost pile on September 9, 2016 at 6:15pm CST.
Naming NotesMy phage name comes from the resemblance between phage tails and squiggles.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 30, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1578
Genome length (bp)68325
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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Alis (None)

Anzano (None)

Azarath (None)

BirdsNest (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

FoxholeBuddy (None)

Indlovu (None)

Kerrigan (None)

LilMcDreamy (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

Alpela (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Benjamin1706 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

Emiris (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Okooko (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShinyBen (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Giraffey (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Apex (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Mithril (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Thonko (B8)

Annotation StatusIn GenBank
Plaque NotesMy phage created clear plaques, with a slight ring of turbidity. The plaques were approximately 1-2mm in diameter after 48 hours of incubation at 30 degrees Celsius.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Squiggle_1
(terminase small subunit)

Squiggle_2
(terminase large subunit)

Squiggle_3

Squiggle_4

Squiggle_5

Squiggle_6
(RuvC-like resolvase)

Squiggle_7

Squiggle_8
(portal protein)

Squiggle_9
(capsid maturation protease)

Squiggle_10
(HNH endonuclease)

Squiggle_11

Squiggle_12
(major capsid protein)

Squiggle_13

Squiggle_14

Squiggle_15

Squiggle_16

Squiggle_17
(membrane protein)

Squiggle_18
(major tail protein)

Squiggle_19

Squiggle_20
(tRNA nucleotidyltransferase)

Squiggle_21

Squiggle_22
(head-to-tail connector complex protein)

Squiggle_23

Squiggle_24

Squiggle_25

Squiggle_26

Squiggle_27

Squiggle_28
(tape measure protein)

Squiggle_29
(minor tail protein)

Squiggle_30
(minor tail protein)

Squiggle_31
(minor tail protein)

Squiggle_32
(minor tail protein)

Squiggle_33
(minor tail protein)

Squiggle_34
(minor tail protein)

Squiggle_35

Squiggle_36

Squiggle_37

Squiggle_38

Squiggle_39

Squiggle_40

Squiggle_41

Squiggle_42

Squiggle_43

Squiggle_44

Squiggle_45
(helix-turn-helix DNA binding domain protein)

Squiggle_46
(helix-turn-helix DNA binding domain protein)

Squiggle_47

Squiggle_48
(lysin A)

Squiggle_49
(lysin B)

Squiggle_50

Squiggle_51
(DNA helicase)

Squiggle_52
(DNA helicase)

Squiggle_53

Squiggle_54

Squiggle_55

Squiggle_56

Squiggle_57

Squiggle_58

Squiggle_59
(DNA primase/polymerase)

Squiggle_60

Squiggle_61
(DNA polymerase I)

Squiggle_62

Squiggle_63
(recombination directionality factor)

Squiggle_64

Squiggle_65

Squiggle_66

Squiggle_67
(ribbon-helix-helix DNA binding domain protein)

Squiggle_68

Squiggle_69
(RNaseE)

Squiggle_70

Squiggle_71

Squiggle_72

Squiggle_73

Squiggle_74

Squiggle_75

Squiggle_76

Squiggle_77

Squiggle_78

Squiggle_79

Squiggle_80

Squiggle_81

Squiggle_82

Squiggle_83
(HNH endonuclease)

Squiggle_84

Squiggle_85

Squiggle_86

Squiggle_87

Squiggle_88

Squiggle_89

Squiggle_90

Squiggle_91

Squiggle_92

Squiggle_93

Squiggle_94

Squiggle_95

Squiggle_96

Squiggle_97
(membrane protein)

Squiggle_98

Squiggle_99

Squiggle_100

Squiggle_101

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK112553
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUsquiggleCWC
SEA Lysate Titer 7.2 x 10^10 pfu/mL
Date of SEA Lysate Titering Oct 13, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# D6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload