Mycobacterium phage MasterPo
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Detailed Information for Phage MasterPo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJessica Landreth and Katie McCandless
Year Found2019
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature34°C
GPS Coordinates32.4625 N, 94.7261 W Map
Discovery NotesFound in area around 2ft from the edge of a stream on LeTourneau University’s main campus. There was a lot of vegetation surrounding the area and was mostly shaded. There was a lot of insect activity, fallen trees, and litter in the area. A small hole about 3-5 inches deep was dug to access soil without organic matter throughout it. Soil texture was fairly damp to the touch, but still crumbly when dug up.
Naming NotesName was inspired by the movie Kung Fu Panda. We realized just how strong our phage was very early in in the process. After many lab technical difficulties, we learned that Po was able to survive even with half the normal nutrients necessary for his survival. This strength reminded us of the main character, Master Ping Xiao Po, in the Kung Fu Panda movies.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1195
Genome length (bp)67361
Character of genome endsCircularly Permuted
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Blitzen (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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MasterPo_1
(ParB-like nuclease domain)

MasterPo_2
(queuine tRNA-ribosyltransferase)

MasterPo_3
(queosine biosynthesis protein, QueC-like)

MasterPo_4
(queosine biosynthesis protein, QueD-like)

MasterPo_5
(queosine biosynthesis protein, QueE-like)

MasterPo_6
(GTP cyclohydrolase I)

MasterPo_7
(terminase)

MasterPo_8

MasterPo_9
(RuvC-like resolvase)

MasterPo_10

MasterPo_11

MasterPo_12
(portal protein)

MasterPo_13
(capsid maturation protease and MuF-like fusion protein)

MasterPo_14

MasterPo_15
(major capsid protein)

MasterPo_16

MasterPo_17

MasterPo_18

MasterPo_19

MasterPo_20

MasterPo_21
(minor tail protein)

MasterPo_22

MasterPo_23

MasterPo_24
(head-to-tail adaptor)

MasterPo_25

MasterPo_26

MasterPo_27
(tail assembly chaperone)

MasterPo_28
(tail assembly chaperone)

MasterPo_29
(tape measure protein)

MasterPo_30
(minor tail protein)

MasterPo_31
(minor tail protein)

MasterPo_32
(minor tail protein)

MasterPo_33
(minor tail protein)

MasterPo_34

MasterPo_35

MasterPo_36

MasterPo_37

MasterPo_38

MasterPo_39

MasterPo_40

MasterPo_41

MasterPo_42

MasterPo_43
(hydrolase)

MasterPo_44
(helix-turn-helix DNA binding domain)

MasterPo_45
(helix-turn-helix DNA binding domain)

MasterPo_46

MasterPo_47

MasterPo_48
(holin)

MasterPo_49

MasterPo_50

MasterPo_51
(DNA helicase)

MasterPo_52

MasterPo_53

MasterPo_54

MasterPo_55
(DNA primase/helicase)

MasterPo_56

MasterPo_57
(DNA polymerase I)

MasterPo_58

MasterPo_59

MasterPo_60

MasterPo_61

MasterPo_62

MasterPo_63

MasterPo_64

MasterPo_65
(ribbon-helix-helix DNA binding domain)

MasterPo_66

MasterPo_67
(helix-turn-helix DNA binding domain)

MasterPo_68

MasterPo_69

MasterPo_70
(HNH endonuclease)

MasterPo_71
(HNH endonuclease)

MasterPo_72

MasterPo_73

MasterPo_74

MasterPo_75

MasterPo_76

MasterPo_77

MasterPo_78

MasterPo_79

MasterPo_80

MasterPo_81

MasterPo_82
(helix-turn-helix DNA binding domain)

MasterPo_83

MasterPo_84

MasterPo_85

MasterPo_86

MasterPo_87

MasterPo_88

MasterPo_89

MasterPo_90

MasterPo_91

MasterPo_92

MasterPo_93

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT639652
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 101
Pitt Freezer Box Grid# F5
Available Files
GenBank File for PhameratorDownload