Mycobacterium phage Mosaic
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Detailed Information for Phage Mosaic
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJudy Wang and Jayaram Mohan
Year Found2011
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.648888 N, 90.314166 W Map
Discovery NotesCollected at a temperature of approximately 80 degrees Fahrenheit.
Soil was moist and under a layer of mulch.
Collected from a depth of approximately 3 centimeters.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 10, 2013
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage198
Sanger Finishing Reactions2
Genome length (bp)68488
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotation StatusIn GenBank
Plaque NotesThe plaques were small with clear centers and hazy edges. Not always completely circular.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Mosaic_1
(adenylate kinase)

Mosaic_2
(terminase)

Mosaic_3

Mosaic_4

Mosaic_5

Mosaic_6
(RuvC-like resolvase)

Mosaic_7

Mosaic_8
(portal protein)

Mosaic_9
(capsid maturation protease and MuF-like fusion protein)

Mosaic_10

Mosaic_11
(major capsid protein)

Mosaic_12

Mosaic_13

Mosaic_14
(holin)

Mosaic_15
(membrane protein)

Mosaic_16
(membrane protein)

Mosaic_17
(major tail protein)

Mosaic_18

Mosaic_19
(queuine tRNA-ribosyltransferase)

Mosaic_20

Mosaic_21
(head-to-tail adaptor)

Mosaic_22

Mosaic_23

Mosaic_24
(tail assembly chaperone)

Mosaic_25

Mosaic_26

Mosaic_27
(tape measure protein)

Mosaic_28
(minor tail protein)

Mosaic_29
(minor tail protein)

Mosaic_30
(minor tail protein)

Mosaic_31
(minor tail protein)

Mosaic_32
(minor tail protein)

Mosaic_33

Mosaic_34

Mosaic_35

Mosaic_36

Mosaic_37

Mosaic_38

Mosaic_39

Mosaic_40

Mosaic_41

Mosaic_42

Mosaic_43

Mosaic_44
(helix-turn-helix DNA binding domain protein)

Mosaic_45
(helix-turn-helix DNA binding domain protein)

Mosaic_46

Mosaic_47
(lysin A)

Mosaic_48
(lysin B)

Mosaic_49

Mosaic_50

Mosaic_51
(DNA helicase)

Mosaic_52

Mosaic_53

Mosaic_54

Mosaic_55

Mosaic_56

Mosaic_57
(DNA primase/helicase)

Mosaic_58

Mosaic_59
(DNA polymerase I)

Mosaic_60

Mosaic_61

Mosaic_62

Mosaic_63

Mosaic_64

Mosaic_65

Mosaic_66

Mosaic_67

Mosaic_68
(RNAseE)

Mosaic_69

Mosaic_70

Mosaic_71
(phospholipase)

Mosaic_72

Mosaic_73
(helix-turn-helix DNA binding domain protein)

Mosaic_74

Mosaic_75

Mosaic_76

Mosaic_77

Mosaic_78

Mosaic_79

Mosaic_80

Mosaic_81

Mosaic_82
(HNH endonuclease)

Mosaic_83

Mosaic_84

Mosaic_85

Mosaic_86

Mosaic_87

Mosaic_88

Mosaic_89

Mosaic_90

Mosaic_91

Mosaic_92

Mosaic_93

Mosaic_94

Mosaic_95

Mosaic_96

Mosaic_97

Mosaic_98

Mosaic_99

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG925350
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2011WUSLmosaicKH
SEA Lysate Titer 5.12e10
Date of SEA Lysate Titering Oct 4, 2011
Pitt Freezer Box# 31
Pitt Freezer Box Grid# 29
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload