Mycobacterium phage Valjean
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Detailed Information for Phage Valjean
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChristine Atik
Year Found2015
Location FoundBaltimore, MD USA
Finding InstitutionJohns Hopkins University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature36°C
GPS Coordinates39.330833 N, 76.619444 W Map
Discovery NotesThe sample was collected from an empty flowerbed outside of the Fresh Food Café at around 1:30 PM. The temperature at the time was 36.4 degrees Celsius, and I dug around 3 centimeters deep to get my sample.
Naming NotesValjean, a siphoviridae with a very long tail, is named after Jean Valjean, a character in the incredible play Les Miserables. Like my phage, Jean Valjean has a very long "tale;" he goes from being a prisoner who breaks parole, to a great mayor of a town, to a loving father.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2673
Genome length (bp)68560
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Boehler (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe plaques have a small, circular shape, and they are approximately 2 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Valjean_1
(adenylate kinase)

Valjean_2
(terminase)

Valjean_3

Valjean_4

Valjean_5

Valjean_6
(RuvC-like resolvase)

Valjean_7
(membrane protein)

Valjean_8
(portal protein)

Valjean_9
(capsid maturation protease and MuF-like fusion protein)

Valjean_10
(HNH endonuclease)

Valjean_11

Valjean_12
(major capsid protein)

Valjean_13

Valjean_14

Valjean_15
(membrane protein)

Valjean_16
(membrane protein)

Valjean_17
(membrane protein)

Valjean_18
(major tail protein)

Valjean_19

Valjean_20
(queuine tRNA-ribosyltransferase)

Valjean_21

Valjean_22
(head-to-tail adaptor)

Valjean_23

Valjean_24

Valjean_25
(tail assembly chaperone)

Valjean_26

Valjean_27

Valjean_28
(tape measure protein)

Valjean_29
(minor tail protein)

Valjean_30
(minor tail protein)

Valjean_31
(minor tail protein)

Valjean_32
(minor tail protein)

Valjean_33
(minor tail protein)

Valjean_34

Valjean_35

Valjean_36

Valjean_37

Valjean_38

Valjean_39

Valjean_40

Valjean_41

Valjean_42

Valjean_43

Valjean_44
(membrane protein)

Valjean_45
(helix-turn-helix DNA binding domain protein)

Valjean_46
(helix-turn-helix DNA binding domain protein)

Valjean_47

Valjean_48
(lysin A)

Valjean_49
(lysin B)

Valjean_50

Valjean_51

Valjean_52
(DNA helicase)

Valjean_53

Valjean_54

Valjean_55

Valjean_56

Valjean_57
(DNA primase/polymerase)

Valjean_58

Valjean_59

Valjean_60
(DNA polymerase I)

Valjean_61

Valjean_62

Valjean_63

Valjean_64

Valjean_65

Valjean_66

Valjean_67

Valjean_68

Valjean_69
(helix-turn-helix DNA binding protein)

Valjean_70

Valjean_71

Valjean_72

Valjean_73

Valjean_74
(HNH endonuclease)

Valjean_75

Valjean_76

Valjean_77

Valjean_78

Valjean_79

Valjean_80

Valjean_81

Valjean_82

Valjean_83
(HNH endonuclease)

Valjean_84

Valjean_85

Valjean_86

Valjean_87

Valjean_88

Valjean_89

Valjean_90

Valjean_91

Valjean_92

Valjean_93

Valjean_94

Valjean_95

Valjean_96
(membrane protein)

Valjean_97

Valjean_98

Valjean_99

Valjean_100

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279889
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.8 x 10^10
Date of SEA Lysate Titering Oct 27, 2015
Pitt Freezer Box# 71
Pitt Freezer Box Grid# E10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload