Mycobacterium phage Halfpint
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Detailed Information for Phage Halfpint
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesN/A
Found ByMadeline Tinskey
Year Found2023
Location FoundCommerce Township, MI United States of America
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature34°C
GPS Coordinates45.58 N, 83.49 W Map
Discovery NotesThis phage was found in an enriched water sample obtained from North Commerce Lake, Michigan. The sample site was a freshwater lake with seaweed, kelp, and sand. The lake levels and humidity in the air were particularly high on the day this sample was collected as there were recent storms.
Naming NotesMy favorite books growing up were the "Little House on the Prairie" series. The author, Laura Ingalls Wilder, writes often of the endearing nickname her father gave her: "half-pint". I wanted to pay homage to one of the most cherished stories of my youth. Additionally, half-pint indicates a very small person just as my phage plaques are small.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1528
Genome length (bp)68449
Character of genome endsCircularly Permuted
GC Content67.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB3
Cluster Life CycleLytic
Lysogeny NotesPlaques are generally very clear as opposed to turbid, indicating the the phage is probably lytic.
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

Basato (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

BlueHusk (B1)

Bluephacebaby (B1)

Boehler (B1)

Brilliant (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

CampRoach (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Chute (B1)

Cobra (B1)

Colbert (B1)

Coletti (B1)

Cornobble (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Daka (B1)

Dati (B1)

DelRivs (B1)

Derpp (B1)

Dice (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Fozzie (B1)

Frankicide (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gwilliam (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Hartsy (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

JDog (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

LasagnaCat (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

Lopsy (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Lumine (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megamind20 (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MiniBoss (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

MrPhizzler (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Nyala (B1)

Olak (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orfeu (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

Pacifista (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhatLouie (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

Quisquiliae (B1)

RedMaple (B1)

Ricotta (B1)

Riggan (B1)

Rimu (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

Scrick (B1)

SDcharge11 (B1)

Selr12 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Simielle (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swiphy (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Hashim76 (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kaleb (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Ben11 (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Deenasa (B3)

Devonte (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

Halfpint (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Kronus (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phayeta (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SlippinJimmy (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

Austelle (B4)

BobbyK (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JaguarMi (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Mudslide (B4)

Nanao (B4)

Nigel (B4)

Poster (B4)

Prince (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

DigitDog (B5)

Donny (B5)

EastView9101 (B5)

Mysterious (B5)

Nangang (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

SirJeffery (B5)

StinkyVeggy (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Annotating InstitutionHope College
Annotation StatusBeing Annotated (Expected completion by 5/1/2024)
Plaque NotesPlaques are very small (less than 0.5 mm) in size, and clear. This indicates that the phage is probably lytic. The plaques are round in shape and the size of pinpricks.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Halfpint_Draft_1

Halfpint_Draft_2

Halfpint_Draft_3

Halfpint_Draft_4

Halfpint_Draft_5

Halfpint_Draft_6

Halfpint_Draft_7

Halfpint_Draft_8

Halfpint_Draft_9

Halfpint_Draft_10

Halfpint_Draft_11

Halfpint_Draft_12

Halfpint_Draft_13

Halfpint_Draft_14

Halfpint_Draft_15

Halfpint_Draft_16

Halfpint_Draft_17

Halfpint_Draft_18

Halfpint_Draft_19

Halfpint_Draft_20

Halfpint_Draft_21

Halfpint_Draft_22

Halfpint_Draft_23

Halfpint_Draft_24

Halfpint_Draft_25

Halfpint_Draft_26

Halfpint_Draft_27

Halfpint_Draft_28

Halfpint_Draft_29

Halfpint_Draft_30

Halfpint_Draft_31

Halfpint_Draft_32

Halfpint_Draft_33

Halfpint_Draft_34

Halfpint_Draft_35

Halfpint_Draft_36

Halfpint_Draft_37

Halfpint_Draft_38

Halfpint_Draft_39

Halfpint_Draft_40

Halfpint_Draft_41

Halfpint_Draft_42

Halfpint_Draft_43

Halfpint_Draft_44

Halfpint_Draft_45

Halfpint_Draft_46

Halfpint_Draft_47

Halfpint_Draft_48

Halfpint_Draft_49

Halfpint_Draft_50

Halfpint_Draft_51

Halfpint_Draft_52

Halfpint_Draft_53

Halfpint_Draft_54

Halfpint_Draft_55

Halfpint_Draft_56

Halfpint_Draft_57

Halfpint_Draft_58

Halfpint_Draft_59

Halfpint_Draft_60

Halfpint_Draft_61

Halfpint_Draft_62

Halfpint_Draft_63

Halfpint_Draft_64

Halfpint_Draft_65

Halfpint_Draft_66

Halfpint_Draft_67

Halfpint_Draft_68

Halfpint_Draft_69

Halfpint_Draft_70

Halfpint_Draft_71

Halfpint_Draft_72

Halfpint_Draft_73

Halfpint_Draft_74

Halfpint_Draft_75

Halfpint_Draft_76

Halfpint_Draft_77

Halfpint_Draft_78

Halfpint_Draft_79

Halfpint_Draft_80

Halfpint_Draft_81

Halfpint_Draft_82

Halfpint_Draft_83

Halfpint_Draft_84

Halfpint_Draft_85

Halfpint_Draft_86

Halfpint_Draft_87

Halfpint_Draft_88

Halfpint_Draft_89

Halfpint_Draft_90

Halfpint_Draft_91

Halfpint_Draft_92

Halfpint_Draft_93

Halfpint_Draft_94

Halfpint_Draft_95

Halfpint_Draft_96

Halfpint_Draft_97

Halfpint_Draft_98

Halfpint_Draft_99

Halfpint_Draft_100

Halfpint_Draft_101

Halfpint_Draft_102

Halfpint_Draft_103

Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 182
Pitt Freezer Box Grid# F7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload