The Actinobacteriophage Database

Mycobacterium phage Trypo

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Detailed Information for Phage Trypo
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Daniel Cui
Year Found	2012
Location Found	St. Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	38.648611 N, 90.311944 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jun 4, 2013
Sequencing Facility	The McDonnell Genome Institute at Washington University
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	230
Genome length (bp)	68798
Character of genome ends	Circularly Permuted
GC Content	66.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) Blitzen (B1) Bluephacebaby (B1) Boehler (B1) Buckeye (B1) CamL (B1) Cannibal (B1) Carthage (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Cobra (B1) Colbert (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) Daffy (B1) Derpp (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Gophee (B1) Grand2040 (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) Jillium (B1) Jiminy (B1) Kahve (B1) Kailash (B1) Keitherie (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) LemonSlice (B1) Longacauda (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megatron (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Numberten (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orion (B1) OSmaximus (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhenghisKhan (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) RedMaple (B1) Riggan (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Scoot17C (B1) SDcharge11 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) Skippy (B1) Slatt (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Timmi (B1) TomBombadil (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Zenteno07 (B13) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kheth (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17a (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Bernardo (B3) ChaChing (B3) Chandler (B3) Compostia (B3) Corofin (B3) Daisy (B3) Gadjet (B3) Gervas (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Marley1013 (B3) Mortcellus (B3) Morty007 (B3) Nozo (B3) Obutu (B3) OrangeOswald (B3) Phaedrus (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) SynergyX (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) AlanGrant (B4) Apex (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Hangman (B4) Heath (B4) Hydro (B4) JAMaL (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Nigel (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) Donny (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Quesadilla (B9)
Annotating Institution	University of Pittsburgh
Annotation Status	In GenBank
Plaque Notes	Small plaques. Clear in the center with a surrounding cloudy circle.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Trypo_1 (adenylate kinase) Trypo_2 (terminase) Trypo_3 Trypo_4 Trypo_5 Trypo_6 (RuvC-like resolvase) Trypo_7 Trypo_8 (portal protein) Trypo_9 (capsid maturation protease and MuF-like fusion protein) Trypo_10 (HNH endonuclease) Trypo_11 Trypo_12 (major capsid protein) Trypo_13 Trypo_14 Trypo_15 (holin) Trypo_16 Trypo_17 Trypo_18 (major tail protein) Trypo_19 Trypo_20 (queuine tRNA-ribosyltransferase) Trypo_21 Trypo_22 (head-to-tail adaptor) Trypo_23 Trypo_24 Trypo_25 (tail assembly chaperone) Trypo_26 Trypo_27 Trypo_28 (tape measure protein) Trypo_29 (minor tail protein) Trypo_30 (minor tail protein) Trypo_31 (minor tail protein) Trypo_32 (minor tail protein) Trypo_33 (minor tail protein) Trypo_34 Trypo_35 Trypo_36 Trypo_37 Trypo_38 Trypo_39 Trypo_40 Trypo_41 Trypo_42 Trypo_43 Trypo_44 Trypo_45 (helix-turn-helix DNA binding domain protein) Trypo_46 (helix-turn-helix DNA binding domain protein) Trypo_47 Trypo_48 Trypo_49 (lysin A) Trypo_50 (lysin B) Trypo_51 Trypo_52 Trypo_53 (DNA helicase) Trypo_54 Trypo_55 Trypo_56 Trypo_57 Trypo_58 Trypo_59 Trypo_60 (DNA primase/helicase) Trypo_61 Trypo_62 Trypo_63 (DNA polymerase) Trypo_64 Trypo_65 Trypo_66 Trypo_67 Trypo_68 Trypo_69 Trypo_70 Trypo_71 (RNaseE) Trypo_72 Trypo_73 Trypo_74 (phospholipase) Trypo_75 Trypo_76 (helix-turn-helix DNA binding domain protein) Trypo_77 Trypo_78 Trypo_79 Trypo_80 Trypo_81 Trypo_82 Trypo_83 Trypo_84 Trypo_85 (HNH endonuclease) Trypo_86 Trypo_87 Trypo_88 Trypo_89 Trypo_90 Trypo_91 Trypo_92 Trypo_93 Trypo_94 Trypo_95 Trypo_96 Trypo_97 Trypo_98 Trypo_99 Trypo_100 Trypo_101
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MG944223
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2012WUSLTrypoKH
SEA Lysate Titer	7.60e9
Date of SEA Lysate Titering	Oct 2, 2012
Pitt Freezer Box#	42
Pitt Freezer Box Grid#	29
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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