Mycobacterium phage Badfish
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Detailed Information for Phage Badfish
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMatthew Olm
Year Found2011
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.440355 N, 79.948457 W Map
Discovery NotesThe sample was found on the top layer of damp soil under a bush . The temperature was around 30 degrees Fahrenheit.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 29, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage842
Sanger Finishing Reactions15
Genome length (bp)69030
Character of genome endsCircularly Permuted
GC Content66.5%
Sequencing Notesnt dimorphism at position 33113 (see assembly data)
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

Basato (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

BlueHusk (B1)

Bluephacebaby (B1)

Boehler (B1)

Brilliant (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

CampRoach (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Chute (B1)

Cobra (B1)

Colbert (B1)

Coletti (B1)

Cornobble (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Daka (B1)

Dati (B1)

DelRivs (B1)

Derpp (B1)

Dice (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Fozzie (B1)

Frankicide (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gwilliam (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Hartsy (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

JDog (B1)

Jillium (B1)

Jiminy (B1)

Jiraiya (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

LasagnaCat (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

Lopsy (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Lumine (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megamind20 (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MiniBoss (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

MrPhizzler (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Nyala (B1)

Olak (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orfeu (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

Pacifista (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhatLouie (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

Quisquiliae (B1)

RedMaple (B1)

Ricotta (B1)

Riggan (B1)

Rimu (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

Scrick (B1)

SDcharge11 (B1)

Selr12 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

SilverBell (B1)

Simielle (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swiphy (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Thunderbird (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Hashim76 (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kaleb (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Vic9 (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Ben11 (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Deenasa (B3)

Devonte (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

Halfpint (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Kronus (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phayeta (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SlippinJimmy (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

Ahwei (B4)

AlanGrant (B4)

Antihero (B4)

Apex (B4)

Austelle (B4)

BobbyK (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Epah (B4)

Fanaw (B4)

Fortunato (B4)

Frederick (B4)

GinPorsche (B4)

Hally898 (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JaguarMi (B4)

JAMaL (B4)

Lambano (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Mudslide (B4)

Nanao (B4)

Nigel (B4)

Poster (B4)

Prince (B4)

QueenPie (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Xincheng (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

DigitDog (B5)

Donny (B5)

EastView9101 (B5)

KarasumaRum (B5)

Mysterious (B5)

Nangang (B5)

Nehemiah (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Shenyu (B5)

SirJeffery (B5)

StinkyVeggy (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Rinkes (B9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques are turbid, about 3mm in diameter, and have a halo.
MorphotypeSiphoviridae
Number of Genes102
Has been Phamerated?Yes
Gene List

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Badfish_1
(adenylate kinase)

Badfish_2
(terminase)

Badfish_3

Badfish_4

Badfish_5

Badfish_6
(RuvC-like resolvase)

Badfish_7

Badfish_8
(portal protein)

Badfish_9
(capsid maturation protease and MuF-like fusion protein)

Badfish_10
(HNH endonuclease domain protein)

Badfish_11

Badfish_12
(major capsid hexamer protein)

Badfish_13
(major capsid pentamer protein)

Badfish_14

Badfish_15

Badfish_16

Badfish_17

Badfish_18
(major tail protein)

Badfish_19

Badfish_20
(queuine tRNA-ribosyltransferase)

Badfish_21

Badfish_22
(head-to-tail adaptor)

Badfish_23

Badfish_24

Badfish_25
(tail assembly chaperone)

Badfish_26

Badfish_27

Badfish_28
(tape measure protein)

Badfish_29
(minor tail protein)

Badfish_30
(minor tail protein)

Badfish_31
(minor tail protein)

Badfish_32
(minor tail protein)

Badfish_33

Badfish_34

Badfish_35

Badfish_36

Badfish_37

Badfish_38

Badfish_39

Badfish_40

Badfish_41

Badfish_42

Badfish_43

Badfish_44

Badfish_45
(helix-turn-helix DNA binding domain protein)

Badfish_46
(helix-turn-helix DNA binding domain protein)

Badfish_47

Badfish_48

Badfish_49
(lysin A)

Badfish_50
(lysin B)

Badfish_51

Badfish_52
(helicase subunit)

Badfish_53
(DNA helicase)

Badfish_54

Badfish_55

Badfish_56

Badfish_57

Badfish_58

Badfish_59
(DNA primase/helicase)

Badfish_60

Badfish_61
(DNA polymerase I)

Badfish_62

Badfish_63

Badfish_64

Badfish_65

Badfish_66

Badfish_67

Badfish_68

Badfish_69

Badfish_70

Badfish_71
(DNA binding protein)

Badfish_72

Badfish_73

Badfish_74

Badfish_75

Badfish_76

Badfish_77

Badfish_78

Badfish_79

Badfish_80

Badfish_81

Badfish_82

Badfish_83

Badfish_84

Badfish_85
(HNH endonuclease domain protein)

Badfish_86

Badfish_87

Badfish_88

Badfish_89

Badfish_90

Badfish_91

Badfish_92

Badfish_93

Badfish_94

Badfish_95

Badfish_96

Badfish_97

Badfish_98

Badfish_99

Badfish_100

Badfish_101

Badfish_102

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKJ194580
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 1
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload