Mycobacterium phage Phantasmagoria
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Detailed Information for Phage Phantasmagoria
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBilalay Tchadi
Year Found2021
Location FoundWilliamsburg, VA United States
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.270007 N, 76.725418 W Map
Discovery NotesPhantasmagoria was isolated from a soil sample taken from the Matoaka Woods behind the College of William & Mary. Soil was collected from the base of decaying tree trunk identified as a likely source of microbial life.
Naming NotesThe name Phantasmagoria captures the feeling of of fascination and magical wonder evoked while working with the chaotic variation generated by (quasi)living systems. It also references the sublime macabre inherent to the study of phages, a biological entity centered around destruction and exploitation of bacteria, ultimately for the greater good.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2335
Genome length (bp)67046
Character of genome endsCircularly Permuted
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Dati (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

Austelle (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

Prince (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Annotating InstitutionCollege of William & Mary
Annotation StatusQC Complete
Plaque NotesPhantasmagoria phages have a distinct head (diameter of ~60 nm) and tail (~280 nm length X ~15nm width). The phage heads seemed to bunch together when looked at through the electron microscope.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Phantasmagoria_1
(ParB-like nuclease domain)

Phantasmagoria_2
(dpdA-like tRNA-guanine transglycosylase)

Phantasmagoria_3
(PreQ0 pathway, queC-like)

Phantasmagoria_4
(PreQ0 pathway, queD-like)

Phantasmagoria_5
(PreQ0 pathway, queE-like)

Phantasmagoria_6
(folE-like GTP cyclohydrolase I)

Phantasmagoria_7
(terminase)

Phantasmagoria_8

Phantasmagoria_9
(RuvC-like resolvase)

Phantasmagoria_10

Phantasmagoria_11

Phantasmagoria_12
(portal protein)

Phantasmagoria_13
(capsid maturation protease)

Phantasmagoria_14

Phantasmagoria_15
(major capsid hexamer protein)

Phantasmagoria_16
(major capsid pentamer protein)

Phantasmagoria_17

Phantasmagoria_18

Phantasmagoria_19

Phantasmagoria_20

Phantasmagoria_21
(major tail protein)

Phantasmagoria_22

Phantasmagoria_23

Phantasmagoria_24
(head-to-tail adaptor)

Phantasmagoria_25

Phantasmagoria_26

Phantasmagoria_27
(tail assembly chaperone)

Phantasmagoria_28
(tail assembly chaperone)

Phantasmagoria_29
(tape measure protein)

Phantasmagoria_30
(minor tail protein)

Phantasmagoria_31
(minor tail protein)

Phantasmagoria_32
(minor tail protein)

Phantasmagoria_33
(minor tail protein)

Phantasmagoria_34

Phantasmagoria_35

Phantasmagoria_36

Phantasmagoria_37

Phantasmagoria_38

Phantasmagoria_39

Phantasmagoria_40

Phantasmagoria_41

Phantasmagoria_42

Phantasmagoria_43
(hydrolase)

Phantasmagoria_44
(helix-turn-helix DNA binding domain)

Phantasmagoria_45
(helix-turn-helix DNA binding domain)

Phantasmagoria_46

Phantasmagoria_47
(lysin A)

Phantasmagoria_48
(holin)

Phantasmagoria_49

Phantasmagoria_50
(Cas4 family exonuclease)

Phantasmagoria_51
(DNA helicase)

Phantasmagoria_52

Phantasmagoria_53

Phantasmagoria_54

Phantasmagoria_55
(DNA primase/polymerase/helicase)

Phantasmagoria_56

Phantasmagoria_57
(DNA polymerase I)

Phantasmagoria_58

Phantasmagoria_59

Phantasmagoria_60

Phantasmagoria_61

Phantasmagoria_62

Phantasmagoria_63
(ribbon-helix-helix DNA binding domain)

Phantasmagoria_64

Phantasmagoria_65
(helix-turn-helix DNA binding domain)

Phantasmagoria_66

Phantasmagoria_67

Phantasmagoria_68
(helix-turn-helix DNA binding domain)

Phantasmagoria_69
(HNH endonuclease)

Phantasmagoria_70

Phantasmagoria_71
(toxin in toxin/antitoxin system, HicA-like)

Phantasmagoria_72

Phantasmagoria_73

Phantasmagoria_74

Phantasmagoria_75

Phantasmagoria_76

Phantasmagoria_77

Phantasmagoria_78

Phantasmagoria_79

Phantasmagoria_80

Phantasmagoria_81

Phantasmagoria_82

Phantasmagoria_83

Phantasmagoria_84

Phantasmagoria_85

Phantasmagoria_86
(membrane domain)

Phantasmagoria_87

Phantasmagoria_88

Phantasmagoria_89

Phantasmagoria_90

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload