Mycobacterium phage Carthage
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Detailed Information for Phage Carthage
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOliver Massey
Year Found2018
Location FoundAuckland, New Zealand
Finding InstitutionMassey University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates36.745556 S, 174.700833 E Map
Discovery NotesWas found in a compost bin from direct plating. Bin was located behind the Massey University Albany campus cafeteria.
Naming NotesCarthage was chosen as I have and interest in history.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 3, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1461
Genome length (bp)67952
Character of genome endsCircularly Permuted
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotating InstitutionMassey University
Annotation StatusIn GenBank
Plaque NotesClear plaques of medium/small size.

Note;
Appears to mutate to have a smaller morphology. Picking an isolated small plaque yields both medium and small plaques. However, Carthage has been purified so I don't think this is two phages
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Carthage_1
(adenylate kinase)

Carthage_2
(terminase)

Carthage_3

Carthage_4

Carthage_5

Carthage_6
(RuvC-like resolvase)

Carthage_7

Carthage_8
(portal protein)

Carthage_9
(capsid maturation protease and minor capsid protein fusion)

Carthage_10

Carthage_11
(major capsid protein)

Carthage_12

Carthage_13

Carthage_14
(membrane protein)

Carthage_15
(membrane protein)

Carthage_16
(membrane protein)

Carthage_17
(major tail protein)

Carthage_18

Carthage_19

Carthage_20
(head-to-tail adaptor)

Carthage_21

Carthage_22

Carthage_23
(tail assembly chaperone)

Carthage_24

Carthage_25

Carthage_26
(tape measure protein)

Carthage_27
(minor tail protein)

Carthage_28
(minor tail protein)

Carthage_29
(minor tail protein)

Carthage_30
(minor tail protein)

Carthage_31
(minor tail protein)

Carthage_32

Carthage_33

Carthage_34

Carthage_35

Carthage_36

Carthage_37

Carthage_38

Carthage_39

Carthage_40

Carthage_41

Carthage_42

Carthage_43
(helix-turn-helix DNA binding domain protein)

Carthage_44
(helix-turn-helix DNA binding domain protein)

Carthage_45

Carthage_46
(lysin A)

Carthage_47
(lysin B)

Carthage_48

Carthage_49

Carthage_50
(DNA helicase)

Carthage_51

Carthage_52

Carthage_53

Carthage_54

Carthage_55

Carthage_56
(DNA primase/polymerase)

Carthage_57

Carthage_58

Carthage_59
(DNA polymerase I)

Carthage_60

Carthage_61

Carthage_62

Carthage_63

Carthage_64

Carthage_65

Carthage_66

Carthage_67

Carthage_68

Carthage_69

Carthage_70
(RNaseE)

Carthage_71

Carthage_72

Carthage_73

Carthage_74

Carthage_75
(HNH endonuclease)

Carthage_76

Carthage_77

Carthage_78

Carthage_79

Carthage_80

Carthage_81

Carthage_82

Carthage_83

Carthage_84

Carthage_85
(HNH endonuclease)

Carthage_86

Carthage_87

Carthage_88

Carthage_89

Carthage_90

Carthage_91

Carthage_92

Carthage_93

Carthage_94

Carthage_95

Carthage_96

Carthage_97

Carthage_98

Carthage_99

Carthage_100

Carthage_101

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524512
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.7X10^10
Pitt Freezer Box# 72
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload