Mycobacterium phage Schadenfreude
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Detailed Information for Phage Schadenfreude
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOlivia Urso
Year Found2015
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates37.001511 N, 86.429974 W Map
Discovery NotesMy soil sample that contained my phage came from the bank of the Barren River underneath a walking bridge.
Naming NotesThe word "schadenfreude" means pleasure derived from another person's misfortune. In a sense, phages do just that: thrive in another organisms (bacterial cells) misfortune, or death.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 1, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage252
Genome length (bp)68591
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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Alis (None)

Azarath (None)

BirdsNest (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Indlovu (None)

Kerrigan (None)

LilMcDreamy (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

Emiris (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Giraffey (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Thonko (B8)

Annotation StatusIn GenBank
Plaque NotesMy plaques were approximately .4 mm in diameter, very circular, and extremely clear. No turbidity was ever observed when streaking my plates.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Schadenfreude_1
(terminase, small subunit)

Schadenfreude_2
(terminase, large subunit)

Schadenfreude_3

Schadenfreude_4

Schadenfreude_5

Schadenfreude_6
(RuvC-like resolvase)

Schadenfreude_7

Schadenfreude_8
(Portal Protein)

Schadenfreude_9
(Capsid Morphogenesis Protein)

Schadenfreude_10

Schadenfreude_11
(major capsid protein)

Schadenfreude_12

Schadenfreude_13

Schadenfreude_14
(membrane protein)

Schadenfreude_15
(membrane protein)

Schadenfreude_16
(membrane protein)

Schadenfreude_17
(major tail protein)

Schadenfreude_18

Schadenfreude_19
(queuine tRNA-ribosyltransferase)

Schadenfreude_20

Schadenfreude_21
(head-to-tail connector complex protein)

Schadenfreude_22

Schadenfreude_23

Schadenfreude_24

Schadenfreude_25

Schadenfreude_26

Schadenfreude_27
(Tape Measure Protein)

Schadenfreude_28
(minor tail protein)

Schadenfreude_29
(minor tail protein)

Schadenfreude_30
(minor tail protein)

Schadenfreude_31
(Minor Tail Protein)

Schadenfreude_32
(Minor Tail Protein)

Schadenfreude_33

Schadenfreude_34

Schadenfreude_35

Schadenfreude_36

Schadenfreude_37

Schadenfreude_38

Schadenfreude_39

Schadenfreude_40

Schadenfreude_41

Schadenfreude_42

Schadenfreude_43
(membrane protein)

Schadenfreude_44
(helix-turn-helix DNA binding domain)

Schadenfreude_45
(helix-turn-helix DNA binding domain)

Schadenfreude_46

Schadenfreude_47
(Lysin A)

Schadenfreude_48
(Lysin B)

Schadenfreude_49

Schadenfreude_50

Schadenfreude_51

Schadenfreude_52
(Helicase)

Schadenfreude_53

Schadenfreude_54

Schadenfreude_55

Schadenfreude_56

Schadenfreude_57
(DNA primase/polymerase and DNA helicase)

Schadenfreude_58

Schadenfreude_59

Schadenfreude_60
(DNA Polymerase I)

Schadenfreude_61

Schadenfreude_62

Schadenfreude_63

Schadenfreude_64
(membrane protein)

Schadenfreude_65

Schadenfreude_66

Schadenfreude_67

Schadenfreude_68

Schadenfreude_69

Schadenfreude_70
(RNAseE)

Schadenfreude_71

Schadenfreude_72

Schadenfreude_73

Schadenfreude_74

Schadenfreude_75
(I-Ppol endonuclease)

Schadenfreude_76

Schadenfreude_77

Schadenfreude_78

Schadenfreude_79

Schadenfreude_80

Schadenfreude_81

Schadenfreude_82

Schadenfreude_83

Schadenfreude_84
(HNH endonuclease)

Schadenfreude_85

Schadenfreude_86

Schadenfreude_87

Schadenfreude_88

Schadenfreude_89

Schadenfreude_90

Schadenfreude_91

Schadenfreude_92

Schadenfreude_93

Schadenfreude_94

Schadenfreude_95

Schadenfreude_96

Schadenfreude_97

Schadenfreude_98
(membrane protein)

Schadenfreude_99

Schadenfreude_100

Schadenfreude_101

Schadenfreude_102

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH926060
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2015WEKUSchadenfreudeOU
SEA Lysate Titer 8.5 x 10^11
Date of SEA Lysate Titering Oct 16, 2015
Pitt Freezer Box# 27
Pitt Freezer Box Grid# F8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload