Detailed Information for Phage Coffee |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Giordanne Logan |
Year Found | 2016 |
Location Found | Washington, DC USA |
Finding Institution | Howard University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 28°C |
GPS Coordinates | 38.922166 N, 77.018151 W Map |
Discovery Notes | The sample was collected outside the Harriet Tubman Quadrangle at a depth of 6.4 cm. |
Naming Notes | The original plaque that was picked from in my group of two was called "Milo"-the name of a popular breakfast drink in my home country,Jamaica. When the groups separated, my lab partner and I decided that she would keep the name Milo and I would use one that was similar. And thus, the name Coffee was created. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 17, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1198 |
Genome length (bp) | 67481 |
Character of genome ends | Circularly Permuted |
GC Content | 69.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | B |
Subcluster | B2 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Winthrop University |
Annotation Status | In GenBank |
Plaque Notes | The plaques were 1 mm in diameter, circular and clear. |
Morphotype | Siphoviridae |
Number of Genes | 91 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK620898 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.325 x 1011 pfu/ml |
Pitt Freezer Box# |
44 |
Pitt Freezer Box Grid# |
C8 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
GenBank File for Phamerator | Download |