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Mycobacterium phage Coffee
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Detailed Information for Phage Coffee
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGiordanne Logan
Year Found2016
Location FoundWashington, DC USA
Finding InstitutionHoward University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates38.922166 N, 77.018151 W Map
Discovery NotesThe sample was collected outside the Harriet Tubman Quadrangle at a depth of 6.4 cm.
Naming NotesThe original plaque that was picked from in my group of two was called "Milo"-the name of a popular breakfast drink in my home country,Jamaica. When the groups separated, my lab partner and I decided that she would keep the name Milo and I would use one that was similar. And thus, the name Coffee was created.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1198
Genome length (bp)67481
Character of genome endsCircularly Permuted
GC Content69.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Other Cluster Members

Click to View

BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotating InstitutionWinthrop University
Annotation StatusIn GenBank
Plaque NotesThe plaques were 1 mm in diameter, circular and clear.
MorphotypeSiphoviridae
Number of Genes91
Has been Phamerated?Yes
Gene List

Click to View

Coffee_1
(helix-turn-helix DNA binding domain protein)

Coffee_2
(queuine tRNA-ribosyltransferase)

Coffee_3
(QueC-like queosine biosynthesis protein)

Coffee_4
(QueD-like queosine biosynthesis protein)

Coffee_5
(QueE-like queosine biosynthesis protein)

Coffee_6
(GTP cyclohydrolase I)

Coffee_7
(terminase)

Coffee_8

Coffee_9
(RuvC-like resolvase)

Coffee_10

Coffee_11

Coffee_12
(portal protein)

Coffee_13
(capsid morphogenesis protein)

Coffee_14

Coffee_15
(major capsid protein)

Coffee_16

Coffee_17

Coffee_18

Coffee_19

Coffee_20

Coffee_21
(major tail protein)

Coffee_22

Coffee_23

Coffee_24
(head-to-tail adaptor)

Coffee_25

Coffee_26

Coffee_27
(tail assembly chaperone)

Coffee_28
(tail assembly chaperone)

Coffee_29
(tape measure protein)

Coffee_30
(minor tail protein)

Coffee_31
(minor tail protein)

Coffee_32
(minor tail protein)

Coffee_33
(minor tail protein)

Coffee_34

Coffee_35

Coffee_36

Coffee_37

Coffee_38

Coffee_39

Coffee_40

Coffee_41

Coffee_42

Coffee_43

Coffee_44
(helix-turn-helix DNA binding domain protein)

Coffee_45
(helix-turn-helix DNA binding domain protein)

Coffee_46

Coffee_47
(lysin A)

Coffee_48
(holin)

Coffee_49

Coffee_50

Coffee_51
(DNA helicase)

Coffee_52

Coffee_53

Coffee_54

Coffee_55
(DNA primase/polymerase)

Coffee_56

Coffee_57
(DNA polymerase I)

Coffee_58

Coffee_59

Coffee_60

Coffee_61

Coffee_62

Coffee_63

Coffee_64
(ribbon-helix-helix DNA binding domain protein)

Coffee_65

Coffee_66

Coffee_67

Coffee_68

Coffee_69
(helix-turn-helix binding domain protein)

Coffee_70

Coffee_71

Coffee_72

Coffee_73

Coffee_74

Coffee_75

Coffee_76

Coffee_77

Coffee_78

Coffee_79

Coffee_80

Coffee_81

Coffee_82

Coffee_83

Coffee_84

Coffee_85

Coffee_86

Coffee_87

Coffee_88

Coffee_89

Coffee_90

Coffee_91

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK620898
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.325 x 1011 pfu/ml
Pitt Freezer Box# 44
Pitt Freezer Box Grid# C8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload