The Actinobacteriophage Database

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Detailed Information for Phage Coffee
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Giordanne Logan
Year Found	2016
Location Found	Washington, DC USA
Finding Institution	Howard University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	28°C
GPS Coordinates	38.922166 N, 77.018151 W Map
Discovery Notes	The sample was collected outside the Harriet Tubman Quadrangle at a depth of 6.4 cm.
Naming Notes	The original plaque that was picked from in my group of two was called "Milo"-the name of a popular breakfast drink in my home country,Jamaica. When the groups separated, my lab partner and I decided that she would keep the name Milo and I would use one that was similar. And thus, the name Coffee was created.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 17, 2017
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	1198
Genome length (bp)	67481
Character of genome ends	Circularly Permuted
GC Content	69.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B2
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Andre (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) Bluephacebaby (B1) Boehler (B1) Buckeye (B1) Burr (B1) CamL (B1) Cannibal (B1) Carthage (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Cher (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Cobra (B1) Colbert (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) DaddyDaniels (B1) Daffy (B1) Dati (B1) Derpp (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Etaye (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) Freya (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giraffe (B1) Gophee (B1) Grand2040 (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Inverness (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) Jillium (B1) Jiminy (B1) Kahve (B1) Kailash (B1) Katniss (B1) Keitherie (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) LeiMonet (B1) LemonSlice (B1) Longacauda (B1) LostAndPhound (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megatron (B1) Melc17 (B1) MelsMeow (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Nicole21 (B1) Numberten (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orion (B1) Orwigg (B1) OSmaximus (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhenghisKhan (B1) Pherdinand (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) RedMaple (B1) Riggan (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Schueller (B1) Scoot17C (B1) SDcharge11 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) Skippy (B1) Slatt (B1) Soile (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Timmi (B1) TomBombadil (B1) Tomlarah (B1) Toni (B1) Tooj (B1) True (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Usavi (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Virgeve (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Weher20 (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Zenteno07 (B13) Allegro (B2) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Calamitous (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kheth (B2) Lars (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Phantasmagoria (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17a (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Bernardo (B3) Briakila (B3) Casbah (B3) ChaChing (B3) Chandler (B3) Compostia (B3) Corofin (B3) Daisy (B3) Gadjet (B3) Gervas (B3) GlenHope (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) LestyG (B3) Marley1013 (B3) MmasiCarm (B3) Mortcellus (B3) Morty007 (B3) Neos5 (B3) Nozo (B3) Obutu (B3) OrangeOswald (B3) Phaedrus (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) SynergyX (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) AlanGrant (B4) Apex (B4) Austelle (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Hangman (B4) Heath (B4) Hydro (B4) JAMaL (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Nigel (B4) Prince (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) Donny (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Funsized (B9) Knocker (B9) PenguinLover67 (B9) Quesadilla (B9)
Annotating Institution	Winthrop University
Annotation Status	In GenBank
Plaque Notes	The plaques were 1 mm in diameter, circular and clear.
Morphotype	Siphoviridae
Number of Genes	91
Has been Phamerated?	Yes
Gene List	Click to View Coffee_1 (helix-turn-helix DNA binding domain protein) Coffee_2 (queuine tRNA-ribosyltransferase) Coffee_3 (Pre Qo pathway QueC-like protein) Coffee_4 (QueD-like queosine biosynthesis protein) Coffee_5 (QueE-like queosine biosynthesis protein) Coffee_6 (GTP cyclohydrolase I) Coffee_7 (terminase) Coffee_8 Coffee_9 (RuvC-like resolvase) Coffee_10 Coffee_11 Coffee_12 (portal protein) Coffee_13 (MuF-like minor capsid protein) Coffee_14 Coffee_15 (major capsid hexamer protein) Coffee_16 (major capsid pentamer protein) Coffee_17 Coffee_18 Coffee_19 Coffee_20 Coffee_21 (major tail protein) Coffee_22 Coffee_23 Coffee_24 (head-to-tail adaptor) Coffee_25 Coffee_26 Coffee_27 (tail assembly chaperone) Coffee_28 (tail assembly chaperone) Coffee_29 (tape measure protein) Coffee_30 (minor tail protein) Coffee_31 (minor tail protein) Coffee_32 (minor tail protein) Coffee_33 (minor tail protein) Coffee_34 Coffee_35 Coffee_36 Coffee_37 Coffee_38 Coffee_39 Coffee_40 Coffee_41 Coffee_42 Coffee_43 Coffee_44 (helix-turn-helix DNA binding domain protein) Coffee_45 (helix-turn-helix DNA-binding protein) Coffee_46 Coffee_47 (lysin A) Coffee_48 (holin) Coffee_49 Coffee_50 Coffee_51 (DNA helicase) Coffee_52 Coffee_53 Coffee_54 Coffee_55 (DNA primase/helicase) Coffee_56 Coffee_57 (DNA polymerase I) Coffee_58 Coffee_59 Coffee_60 Coffee_61 Coffee_62 Coffee_63 Coffee_64 (ribbon-helix-helix DNA binding domain protein) Coffee_65 Coffee_66 (DNA binding protein) Coffee_67 Coffee_68 Coffee_69 (DNA binding protein) Coffee_70 Coffee_71 Coffee_72 Coffee_73 Coffee_74 Coffee_75 Coffee_76 Coffee_77 Coffee_78 Coffee_79 Coffee_80 Coffee_81 Coffee_82 Coffee_83 Coffee_84 Coffee_85 Coffee_86 Coffee_87 Coffee_88 Coffee_89 Coffee_90 Coffee_91
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK620898
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	1.325 x 1011 pfu/ml
Pitt Freezer Box#	44
Pitt Freezer Box Grid#	C8
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
GenBank File for Phamerator	Download

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