The Actinobacteriophage Database

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Detailed Information for Phage Coffee
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Giordanne Logan
Year Found	2016
Location Found	Washington, DC United States of America
Finding Institution	Howard University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	28°C
GPS Coordinates	38.922166 N, 77.018151 W Map
Discovery Notes	The sample was collected outside the Harriet Tubman Quadrangle at a depth of 6.4 cm.
Naming Notes	The original plaque that was picked from in my group of two was called "Milo"-the name of a popular breakfast drink in my home country,Jamaica. When the groups separated, my lab partner and I decided that she would keep the name Milo and I would use one that was similar. And thus, the name Coffee was created.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 17, 2017
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	1198
Genome length (bp)	67481
Character of genome ends	Circularly Permuted
GC Content	69.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B2
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Alis (None) Anzano (None) Azarath (None) BirdsNest (None) BlaqueIvy (None) Cally (None) Ceci11 (None) ChrIke (None) Conway (None) CptKIRK (None) DaHileesi (None) FoxholeBuddy (None) Indlovu (None) Kerrigan (None) LilMcDreamy (None) Loadofcarp (None) Mage (None) Meraxes (None) Muus (None) Olive00 (None) Pallavicini (None) RoseLilly (None) SeTi1 (None) Skye (None) Thalatta (None) Themis (None) TommyBoy (None) ABU (B1) Alex (B1) Alpela (B1) AltPhacts (B1) Altwerkus (B1) Antonia (B1) Apizium (B1) ArigunDD10 (B1) Ashraf (B1) AstrumP28 (B1) AyJay1824 (B1) Badfish (B1) Banjo (B1) BatteryCK (B1) Beaglebox (B1) BengaliBalla92 (B1) Beni05 (B1) Benjamin1706 (B1) Bishoperium (B1) BlackStallion (B1) BRod943 (B1) Buckeye (B1) BullsEye (B1) CamL (B1) Cannibal (B1) Carthage (B1) Ceraveeno (B1) Chah (B1) CharlieGBrown (B1) CheetO (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Cobra (B1) Coffean (B1) Colbert (B1) Cold1 (B1) Cosmolli16 (B1) Cowgirl (B1) Craff (B1) Crownjwl (B1) Daffy (B1) Derpp (B1) Dingo (B1) Dione (B1) Ditto (B1) Doc (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) DuffyC (B1) Durga (B1) Egahp (B1) Emiris (B1) EmmyMG (B1) EmpTee (B1) Eremos (B1) Eugenia (B1) Fang (B1) Ferhersher (B1) FluffyNinja (B1) Frankicide (B1) FriarPreacher (B1) Fringe (B1) Frogger (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giddykiddy (B1) Girly (B1) Gophee (B1) Grand2040 (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inkblot (B1) IPP (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) Jbone (B1) Jillium (B1) Josephine (B1) Kahve (B1) Kailash (B1) Kat63 (B1) Keitherie (B1) Kikipoo (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) Lasso (B1) Lauryn (B1) LeeLot (B1) Legion (B1) Lego3393 (B1) Legolas (B1) LemonSlice (B1) Lifestyle (B1) Llorona (B1) Longacauda (B1) LoveandHonor (B1) LuckyMarjie (B1) Lulumae (B1) Mag7 (B1) Maleldil (B1) Malkin71 (B1) Mana (B1) Manad (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megatron (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) Mistert4 (B1) MitKao (B1) Morbach (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Novak (B1) Numberten (B1) Okooko (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orion (B1) OSmaximus (B1) Pacos19 (B1) PDRPv (B1) Pearsonia (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhenghisKhan (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Pickrel13 (B1) Piglet (B1) PinheadLarry (B1) PinkLady16 (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prann (B1) ProfessorX (B1) Puhltonio (B1) PuraVida (B1) Pyro (B1) QueenBeane (B1) RedMaple (B1) Riggan (B1) Robyn (B1) Rolex (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Scoot17C (B1) Scooterbug (B1) SDcharge11 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShinyBen (B1) ShiVal (B1) Sigman (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Speckles (B1) Squid (B1) Squiggle (B1) Squirt12 (B1) Steve27 (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swish (B1) TallGrassMM (B1) Thora (B1) ThreeOh3D2 (B1) Tigris (B1) Timmi (B1) TomBombadil (B1) Tre (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) Ulysses2016 (B1) UncleHowie (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Vista (B1) Vivaldi (B1) Vivamus (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Wunderlich (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Ypsitucky (B1) Zaider (B1) Zelda (B1) Zonia (B1) Arbiter (B2) Ares (B2) Boyle (B2) Brownie5 (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Julius (B2) Kheth (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) Opia (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17a (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Bernardo (B3) ChaChing (B3) Chandler (B3) CharlesTrout (B3) Compostia (B3) Corofin (B3) Daisy (B3) Gadjet (B3) Giraffey (B3) Gulsi (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Littleengine (B3) Marley1013 (B3) Mortcellus (B3) Morty007 (B3) Nozo (B3) OrangeOswald (B3) Phaedrus (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) RomaT (B3) SassBadger (B3) Tydolla (B3) Yahalom (B3) Zollars18 (B3) Adawi (B4) AlanGrant (B4) Apex (B4) Bane1 (B4) Bane2 (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Halloween (B4) Hangman (B4) JAMaL (B4) LeapingLizzy (B4) Mithril (B4) Nigel (B4) Paliothales9 (B4) RawrgerThat (B4) Skruzzles (B4) Stinger (B4) TheRipper (B4) Tvorc (B4) Vincenzo (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) Donny (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Larry28 (B7) Saguaro (B7) Tapir (B7) Thonko (B8)
Annotation Status	Being Annotated (Expected completion by 4/8/2019)
Plaque Notes	The plaques were 1 mm in diameter, circular and clear.
Morphotype	Siphoviridae
Number of Genes	91
Has been Phamerated?	Yes
Gene List	Click to View Coffee_1 Coffee_2 Coffee_3 Coffee_4 Coffee_5 Coffee_6 Coffee_7 Coffee_8 Coffee_9 Coffee_10 Coffee_11 Coffee_12 Coffee_13 Coffee_14 Coffee_15 Coffee_16 Coffee_17 Coffee_18 Coffee_19 Coffee_20 Coffee_21 Coffee_22 Coffee_23 Coffee_24 Coffee_25 Coffee_26 Coffee_27 Coffee_28 Coffee_29 Coffee_30 Coffee_31 Coffee_32 Coffee_33 Coffee_34 Coffee_35 Coffee_36 Coffee_37 Coffee_38 Coffee_39 Coffee_40 Coffee_41 Coffee_42 Coffee_43 Coffee_44 Coffee_45 Coffee_46 Coffee_47 Coffee_48 Coffee_49 Coffee_50 Coffee_51 Coffee_52 Coffee_53 Coffee_54 Coffee_55 Coffee_56 Coffee_57 Coffee_58 Coffee_59 Coffee_60 Coffee_61 Coffee_62 Coffee_63 Coffee_64 Coffee_65 Coffee_66 Coffee_67 Coffee_68 Coffee_69 Coffee_70 Coffee_71 Coffee_72 Coffee_73 Coffee_74 Coffee_75 Coffee_76 Coffee_77 Coffee_78 Coffee_79 Coffee_80 Coffee_81 Coffee_82 Coffee_83 Coffee_84 Coffee_85 Coffee_86 Coffee_87 Coffee_88 Coffee_89 Coffee_90 Coffee_91
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	1.325 x 1011 pfu/ml
Pitt Freezer Box#	44
Pitt Freezer Box Grid#	C8
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download

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