| Detailed Information for Phage Coffee |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Giordanne Logan |
| Year Found | 2016 |
| Location Found | Washington, DC USA |
| Finding Institution | Howard University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 38.922166 N, 77.018151 W Map |
| Discovery Notes | The sample was collected outside the Harriet Tubman Quadrangle at a depth of 6.4 cm. |
| Naming Notes | The original plaque that was picked from in my group of two was called "Milo"-the name of a popular breakfast drink in my home country,Jamaica. When the groups separated, my lab partner and I decided that she would keep the name Milo and I would use one that was similar. And thus, the name Coffee was created. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1198 |
| Genome length (bp) | 67481 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Winthrop University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were 1 mm in diameter, circular and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK620898 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.325 x 1011 pfu/ml |
| Pitt Freezer Box# |
44 |
| Pitt Freezer Box Grid# |
C8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
