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Mycobacterium phage Sheila

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Detailed Information for Phage Sheila
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Teams Red Hot Chili Phages & Bringing Sexy Plaque
Year Found	2015
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	35°C
GPS Coordinates	34.070833 N, 118.450833 W Map
Discovery Notes	Soil sample was collected by Emily Xu from UCLA Dormitories, De Neve Evergreen. From moist soil, near bushes from a depth of 5cm. Sample taken from shaded area, little sunlight. Soil sample was Enriched in 7H9 Glycerol Medium, AD Supplement, 100mM CaCl2, and 48hr culture of M. smeg. Incubated at 35oC for 24hrs. Phage Sheila was isolated,characterized, and annotated by Team Red Hot Chili Phages and Team Bringing Sexy Plaque. Red Hot Chili Phages: Emily Xu, Stella Joh, Ximena Ibarra, Nabil Aziz Bringing Sexy Plaque: Marina Krygin, Jacob Lui, Andrew Lund, Kathryn Rosenbaum TA: Emma Goodwin Instructor: Jordan Moberg Parker
Naming Notes	One team member once had a golden retriever named Sheila. She had a long tail.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Sep 11, 2015
Sequencing Facility	NC State Genomic Sciences Laboratory
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	4491
Genome length (bp)	67880
Character of genome ends	Circularly Permuted
GC Content	66.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B1
Cluster Life Cycle	Lytic
Lysogeny Notes	Lytic phage. Plaques displayed increasingly clear morphology over time and Sheila failed to produce mesas in the lysogeny test. Sheila's genome lacks the characteristic lysogenic gene, Integrase.
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Andre (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) Bluephacebaby (B1) Boehler (B1) Buckeye (B1) Burr (B1) CamL (B1) Cannibal (B1) Carthage (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Cher (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Cobra (B1) Colbert (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) DaddyDaniels (B1) Daffy (B1) Dati (B1) Derpp (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Etaye (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) Freya (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giraffe (B1) Gophee (B1) Grand2040 (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Inverness (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) Jillium (B1) Jiminy (B1) Kahve (B1) Kailash (B1) Katniss (B1) Keitherie (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) LeiMonet (B1) LemonSlice (B1) Longacauda (B1) LostAndPhound (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megatron (B1) Melc17 (B1) MelsMeow (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Nicole21 (B1) Numberten (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orion (B1) Orwigg (B1) OSmaximus (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhenghisKhan (B1) Pherdinand (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) RedMaple (B1) Riggan (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Schueller (B1) Scoot17C (B1) SDcharge11 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) Skippy (B1) Slatt (B1) Soile (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Timmi (B1) TomBombadil (B1) Tomlarah (B1) Toni (B1) Tooj (B1) True (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Usavi (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Virgeve (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Weher20 (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Zenteno07 (B13) Allegro (B2) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Calamitous (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kheth (B2) Lars (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Phantasmagoria (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17a (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Bernardo (B3) Briakila (B3) Casbah (B3) ChaChing (B3) Chandler (B3) Compostia (B3) Corofin (B3) Daisy (B3) Gadjet (B3) Gervas (B3) GlenHope (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) LestyG (B3) Marley1013 (B3) MmasiCarm (B3) Mortcellus (B3) Morty007 (B3) Neos5 (B3) Nozo (B3) Obutu (B3) OrangeOswald (B3) Phaedrus (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) SynergyX (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) AlanGrant (B4) Apex (B4) Austelle (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Hangman (B4) Heath (B4) Hydro (B4) JAMaL (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Nigel (B4) Prince (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) Donny (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Funsized (B9) Knocker (B9) PenguinLover67 (B9) Quesadilla (B9)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Produced both Clear plaques and plaques with turbid edges and clear centers. Average size: 3.9mm +/- 0.6mm
Morphotype	Siphoviridae
Number of Genes	96
Has been Phamerated?	Yes
Gene List	Click to View Sheila_1 (adenylate kinase) Sheila_2 (terminase) Sheila_3 Sheila_4 Sheila_5 Sheila_6 (RuvC-like resolvase) Sheila_7 Sheila_8 (portal protein) Sheila_9 (capsid maturation protease and MuF-like fusion protein) Sheila_10 (HNH endonuclease) Sheila_11 Sheila_12 (major capsid hexamer protein) Sheila_13 (major capsid pentamer protein) Sheila_14 Sheila_15 Sheila_16 Sheila_17 Sheila_18 (major tail protein) Sheila_19 Sheila_20 (queuine tRNA-ribosyltransferase) Sheila_21 Sheila_22 (head-to-tail adaptor) Sheila_23 Sheila_24 Sheila_25 (tail assembly chaperone) Sheila_26 Sheila_27 Sheila_28 (tape measure protein) Sheila_29 (minor tail protein) Sheila_30 (minor tail protein) Sheila_31 (minor tail protein) Sheila_32 (minor tail protein) Sheila_33 (minor tail protein) Sheila_34 Sheila_35 Sheila_36 Sheila_37 Sheila_38 Sheila_39 Sheila_40 Sheila_41 Sheila_42 Sheila_43 Sheila_44 Sheila_45 (helix-turn-helix DNA binding domain protein) Sheila_46 (helix-turn-helix DNA binding domain protein) Sheila_47 Sheila_48 (lysin A) Sheila_49 (lysin B) Sheila_50 Sheila_51 Sheila_52 (DNA helicase) Sheila_53 Sheila_54 Sheila_55 Sheila_56 (DNA primase/helicase) Sheila_57 Sheila_58 (DNA polymerase I) Sheila_59 Sheila_60 Sheila_61 Sheila_62 Sheila_63 Sheila_64 Sheila_65 Sheila_66 Sheila_67 Sheila_68 Sheila_69 Sheila_70 Sheila_71 Sheila_72 Sheila_73 Sheila_74 Sheila_75 Sheila_76 Sheila_77 Sheila_78 Sheila_79 Sheila_80 (HNH endonuclease) Sheila_81 Sheila_82 Sheila_83 Sheila_84 Sheila_85 Sheila_86 Sheila_87 Sheila_88 Sheila_89 Sheila_90 Sheila_91 Sheila_92 Sheila_93 Sheila_94 Sheila_95 Sheila_96
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF919530
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	23
Pitt Freezer Box Grid#	E9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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