Mycobacterium phage Sheila
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Detailed Information for Phage Sheila
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeams Red Hot Chili Phages & Bringing Sexy Plaque
Year Found2015
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.070833 N, 118.450833 W Map
Discovery NotesSoil sample was collected by Emily Xu from UCLA Dormitories, De Neve Evergreen. From moist soil, near bushes from a depth of 5cm. Sample taken from shaded area, little sunlight. Soil sample was Enriched in 7H9 Glycerol Medium, AD Supplement, 100mM  CaCl2, and 48hr culture of  M. smeg. Incubated at 35oC for 24hrs.

Phage Sheila was isolated,characterized, and annotated by Team Red Hot Chili Phages and Team Bringing Sexy Plaque.

Red Hot Chili Phages: Emily Xu, Stella Joh, Ximena Ibarra, Nabil Aziz
Bringing Sexy Plaque: Marina Krygin, Jacob Lui, Andrew Lund, Kathryn Rosenbaum
TA: Emma Goodwin
Instructor: Jordan Moberg Parker
Naming NotesOne team member once had a golden retriever named Sheila. She had a long tail.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 11, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4491
Genome length (bp)67880
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Lysogeny NotesLytic phage. Plaques displayed increasingly clear morphology over time and Sheila failed to produce mesas in the lysogeny test. Sheila's genome lacks the characteristic lysogenic gene, Integrase.
Other Cluster Members

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BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

Quesadilla (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesProduced both Clear plaques and plaques with turbid edges and clear centers.
Average size: 3.9mm +/- 0.6mm
MorphotypeSiphoviridae
Number of Genes96
Has been Phamerated?Yes
Gene List

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Sheila_1
(adenylate kinase)

Sheila_2
(terminase)

Sheila_3

Sheila_4

Sheila_5

Sheila_6
(RuvC-like resolvase)

Sheila_7

Sheila_8
(portal protein)

Sheila_9
(capsid maturation protease and MuF-like fusion protein)

Sheila_10
(HNH endonuclease)

Sheila_11

Sheila_12
(major capsid protein)

Sheila_13

Sheila_14

Sheila_15

Sheila_16

Sheila_17

Sheila_18
(major tail protein)

Sheila_19

Sheila_20
(queuine tRNA-ribosyltransferase)

Sheila_21

Sheila_22
(head-to-tail adaptor)

Sheila_23

Sheila_24

Sheila_25
(tail assembly chaperone)

Sheila_26

Sheila_27

Sheila_28
(tape measure protein)

Sheila_29
(minor tail protein)

Sheila_30
(minor tail protein)

Sheila_31
(minor tail protein)

Sheila_32
(minor tail protein)

Sheila_33
(minor tail protein)

Sheila_34

Sheila_35

Sheila_36

Sheila_37

Sheila_38

Sheila_39

Sheila_40

Sheila_41

Sheila_42

Sheila_43

Sheila_44

Sheila_45
(helix-turn-helix DNA binding domain protein)

Sheila_46
(helix-turn-helix DNA binding domain protein)

Sheila_47

Sheila_48
(lysin A)

Sheila_49
(lysin B)

Sheila_50

Sheila_51

Sheila_52
(DNA helicase)

Sheila_53

Sheila_54

Sheila_55

Sheila_56
(DNA primase/polymerase)

Sheila_57

Sheila_58
(DNA polymerase I)

Sheila_59

Sheila_60

Sheila_61

Sheila_62

Sheila_63

Sheila_64

Sheila_65

Sheila_66
(endoribonuclease)

Sheila_67

Sheila_68

Sheila_69

Sheila_70

Sheila_71
(helix-turn-helix DNA binding domain protein)

Sheila_72

Sheila_73

Sheila_74

Sheila_75

Sheila_76

Sheila_77

Sheila_78

Sheila_79

Sheila_80
(HNH endonuclease)

Sheila_81

Sheila_82

Sheila_83

Sheila_84

Sheila_85

Sheila_86

Sheila_87

Sheila_88

Sheila_89

Sheila_90

Sheila_91

Sheila_92

Sheila_93

Sheila_94

Sheila_95

Sheila_96

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919530
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 23
Pitt Freezer Box Grid# E9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload