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Mycobacterium phage Sabella
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Detailed Information for Phage Sabella
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMariam Dar
Year Found2016
Location FoundNorth Brunswick, NJ USA
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.445858 N, 74.52067 W Map
Discovery NotesThe moist soil sample was taken 2.5 cm deep from the organic vegetable patch in my backyard, where tomato plants are grown.
Naming NotesThe local park in North Brunswick is called Sabella Park, where I searched for soil samples this summer. As a child, I remember loving the swings and playing soccer at this park, so I named my phage "sabella".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 16, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage164
Genome length (bp)67304
Character of genome endsCircularly Permuted
GC Content68.9%
Sequencing NotesThis genome was sequenced as part of a DOGEMS sample.
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Other Cluster Members

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BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusIn GenBank
Plaque NotesSabella has two different morphologies: some of the plaques are 0.1 cm in diameter, while others are 0.15 cm in diameter. This notable difference was tested multiple times, so the phage population of this lytic phage is consistent and contains two morphologies. All the plaques are clear and round with smooth edges.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Sabella_1

Sabella_2

Sabella_3
(QueC-like queuosine biosynthesis protein)

Sabella_4
(QueD-like queuosine biosynthesis protein)

Sabella_5
(QueE-like queuosine biosynthesis protein)

Sabella_6
(hydrolase)

Sabella_7
(terminase)

Sabella_8

Sabella_9
(RuvC-like resolvase)

Sabella_10

Sabella_11

Sabella_12
(portal protein)

Sabella_13
(capsid maturation protease and MuF-like fusion protein)

Sabella_14

Sabella_15

Sabella_16

Sabella_17

Sabella_18

Sabella_19

Sabella_20

Sabella_21
(major tail protein)

Sabella_22

Sabella_23

Sabella_24
(head-to-tail adaptor)

Sabella_25

Sabella_26

Sabella_27
(tail assembly chaperone)

Sabella_28

Sabella_29
(tape measure protein)

Sabella_30
(minor tail protein)

Sabella_31
(minor tail protein)

Sabella_32
(minor tail protein)

Sabella_33

Sabella_34

Sabella_35

Sabella_36

Sabella_37

Sabella_38

Sabella_39

Sabella_40

Sabella_41

Sabella_42

Sabella_43
(helix-turn-helix DNA binding domain protein)

Sabella_44
(helix-turn-helix DNA binding domain protein)

Sabella_45

Sabella_46
(lysin A)

Sabella_47

Sabella_48

Sabella_49

Sabella_50
(helicase)

Sabella_51

Sabella_52

Sabella_53

Sabella_54
(helicase)

Sabella_55

Sabella_56
(DNA polymerase)

Sabella_57

Sabella_58

Sabella_59

Sabella_60

Sabella_61

Sabella_62

Sabella_63

Sabella_64

Sabella_65

Sabella_66
(helix-turn-helix DNA binding domain protein)

Sabella_67

Sabella_68

Sabella_69

Sabella_70
(HNH endonuclease)

Sabella_71

Sabella_72

Sabella_73

Sabella_74

Sabella_75

Sabella_76

Sabella_77

Sabella_78

Sabella_79

Sabella_80

Sabella_81

Sabella_82

Sabella_83

Sabella_84

Sabella_85

Sabella_86

Sabella_87

Sabella_88

Sabella_89

Sabella_90

Sabella_91

Sabella_92

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH590591
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.7*10^10 pfu/mL
Date of SEA Lysate Titering Oct 4, 2016
Pitt Freezer Box# 34
Pitt Freezer Box Grid# F9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload