Mycobacterium phage Magic8
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Detailed Information for Phage Magic8
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAnne Winkler
Year Found2019
Location FoundPhiladelphia, PA USA
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates39.996 N, 75.231 W Map
Discovery NotesFound on October 10, 2019. The soil it was collected from was moist and a little chalky. The humidity was 47%.
Naming NotesIt was my 8th sample I collected in the process so I named Magic8 like a magic 8 ball.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1559
Genome length (bp)68865
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Dati (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

Austelle (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

Prince (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Annotating InstitutionSaint Joseph's University
Annotation StatusIn GenBank
Plaque NotesThe plaques are clear so it is lytic.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Magic8_1
(adenylate kinase)

Magic8_2
(terminase)

Magic8_3

Magic8_4

Magic8_5

Magic8_6
(RuvC-like resolvase)

Magic8_7

Magic8_8
(portal protein)

Magic8_9
(capsid maturation protease and MuF-like fusion protein)

Magic8_10
(HNH endonuclease)

Magic8_11

Magic8_12
(major capsid hexamer protein)

Magic8_13
(major capsid pentamer protein)

Magic8_14

Magic8_15
(holin)

Magic8_16

Magic8_17

Magic8_18
(major tail protein)

Magic8_19

Magic8_20
(queuine tRNA-ribosyltransferase)

Magic8_21

Magic8_22
(head-to-tail adaptor)

Magic8_23

Magic8_24

Magic8_25
(tail assembly chaperone)

Magic8_26

Magic8_27

Magic8_28
(tape measure protein)

Magic8_29
(minor tail protein)

Magic8_30
(minor tail protein)

Magic8_31
(minor tail protein)

Magic8_32
(minor tail protein)

Magic8_33
(minor tail protein)

Magic8_34

Magic8_35

Magic8_36

Magic8_37

Magic8_38

Magic8_39

Magic8_40

Magic8_41

Magic8_42

Magic8_43

Magic8_44

Magic8_45
(helix-turn-helix DNA binding domain protein)

Magic8_46
(helix-turn-helix DNA binding domain protein)

Magic8_47

Magic8_48
(lysin A)

Magic8_49
(lysin B)

Magic8_50

Magic8_51

Magic8_52
(DNA helicase)

Magic8_53

Magic8_54

Magic8_55

Magic8_56

Magic8_57

Magic8_58

Magic8_59
(DNA primase/polymerase)

Magic8_60

Magic8_61
(DNA polymerase I)

Magic8_62

Magic8_63

Magic8_64

Magic8_65

Magic8_66

Magic8_67

Magic8_68

Magic8_69
(RNaseE)

Magic8_70

Magic8_71

Magic8_72

Magic8_73

Magic8_74
(HNH endonuclease)

Magic8_75

Magic8_76

Magic8_77

Magic8_78

Magic8_79

Magic8_80

Magic8_81

Magic8_82

Magic8_83
(HNH endonuclease)

Magic8_84

Magic8_85

Magic8_86

Magic8_87

Magic8_88

Magic8_89

Magic8_90

Magic8_91

Magic8_92

Magic8_93

Magic8_94

Magic8_95

Magic8_96

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT776813
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.2 x 10^10 pfu/mL
Pitt Freezer Box# 101
Pitt Freezer Box Grid# G8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload