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Mycobacterium phage Giraffe
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Detailed Information for Phage Giraffe
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKate Lowrey and Alyssa Christoffer
Year Found2014
Location FoundWest Lafayette, IN USA
Finding InstitutionPurdue University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.421344 N, 86.922364 E Map
Discovery Notes2cm deep
moist soil
Landscape Garden
Naming NotesIt has a very Long tail so we decided it was best depicted by a giraffe

Re-amplified and purified in 2020 by Maansi Asthana, Anita Gopalrathnam, Victoria Granat, David Schaeper, Holly Weilbaker, Brendan Williams
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1072
Genome length (bp)68869
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Dati (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

Prince (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Annotating InstitutionPurdue University
Annotation StatusIn GenBank
Plaque NotesThe plaque that was captured at Discovery Learning Research Center is a phage that forms plaques with an average diameter of 4mm. The plaques are very clear which indicates the phage induces lysis of its host. The phage grows rapidly and often takes over the plate, when it is in a concentrated area the plaque’s diameter decreases because of space constraints.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Giraffe_1
(adenylate kinase)

Giraffe_2
(terminase)

Giraffe_3

Giraffe_4

Giraffe_5

Giraffe_6
(RuvC-like resolvase)

Giraffe_7

Giraffe_8
(portal protein)

Giraffe_9
(capsid maturation protease and MuF-like fusion protein)

Giraffe_10

Giraffe_11

Giraffe_12
(major capsid protein)

Giraffe_13

Giraffe_14

Giraffe_15

Giraffe_16

Giraffe_17

Giraffe_18
(major tail protein)

Giraffe_19

Giraffe_20
(queuine tRNA-ribosyltransferase)

Giraffe_21

Giraffe_22
(head-to-tail adaptor)

Giraffe_23

Giraffe_24

Giraffe_25
(tail assembly chaperone)

Giraffe_26

Giraffe_27

Giraffe_28
(tape measure protein)

Giraffe_29
(minor tail protein)

Giraffe_30
(minor tail protein)

Giraffe_31
(minor tail protein)

Giraffe_32
(minor tail protein)

Giraffe_33
(minor tail protein)

Giraffe_34

Giraffe_35

Giraffe_36

Giraffe_37

Giraffe_38

Giraffe_39

Giraffe_40

Giraffe_41

Giraffe_42

Giraffe_43

Giraffe_44

Giraffe_45
(helix-turn-helix DNA binding domain protein)

Giraffe_46
(helix-turn-helix DNA binding domain protein)

Giraffe_47

Giraffe_48
(lysin A)

Giraffe_49
(lysin B)

Giraffe_50

Giraffe_51

Giraffe_52
(DNA helicase)

Giraffe_53

Giraffe_54

Giraffe_55

Giraffe_56

Giraffe_57

Giraffe_58

Giraffe_59
(DNA primase/helicase)

Giraffe_60

Giraffe_61
(DNA polymerase I)

Giraffe_62

Giraffe_63

Giraffe_64

Giraffe_65

Giraffe_66

Giraffe_67

Giraffe_68

Giraffe_69

Giraffe_70
(helix-turn-helix DNA binding domain protein)

Giraffe_71

Giraffe_72

Giraffe_73

Giraffe_74

Giraffe_75

Giraffe_76

Giraffe_77

Giraffe_78

Giraffe_79

Giraffe_80

Giraffe_81

Giraffe_82

Giraffe_83

Giraffe_84
(HNH endonuclease)

Giraffe_85

Giraffe_86

Giraffe_87

Giraffe_88

Giraffe_89

Giraffe_90

Giraffe_91

Giraffe_92

Giraffe_93

Giraffe_94

Giraffe_95

Giraffe_96

Giraffe_97

Giraffe_98

Giraffe_99

Giraffe_100

Giraffe_101

Giraffe_102

Giraffe_103

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ622172
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.9x10^9
Pitt Freezer Box# 12
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload