Mycobacterium phage Roscoe
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Detailed Information for Phage Roscoe
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJacob Bordeaux
Year Found2013
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.786246 N, 86.101972 W Map
Discovery NotesSoil sample was taken from moist soil approximately 3 cm deep. The sample was taken from a landscaping area next to a sidewalk and building.
Naming NotesRoscoe is one of my dog's name
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage318
Sanger Finishing Reactions11
Genome length (bp)68603
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe plaque size varies between 2mm-4mm. All plaques have clear area of lysis and most show an asymmetrical ring of turbidity around the outside of the clear portion. Roscoe grows at 30, 37, and 42 degrees Celsius. At 37, the average plaque size is 1-2mm larger than when grown at 30 but the turbid ring is the same size. At 42, the average plaque size increases by another 1-2mm from 37, and the turbid ring becomes much larger. When tested at 30 degrees with varying concentrations of CaCl2 in top augur and bacterial cells, no change in growth was noticed. At high enough concentrations, Roscoe shows a density dependent effect observed as a multitude of pinprick sized plaques covering the plate with a number of larger plaques throughout.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Roscoe_1
(adenylate kinase)

Roscoe_2

Roscoe_3
(terminase)

Roscoe_4

Roscoe_5

Roscoe_6

Roscoe_7
(RuvC-like resolvase)

Roscoe_8

Roscoe_9
(portal protein)

Roscoe_10
(capsid maturation protease and MuF-like fusion protein)

Roscoe_11

Roscoe_12

Roscoe_13
(major capsid protein)

Roscoe_14

Roscoe_15

Roscoe_16
(membrane protein)

Roscoe_17
(membrane protein)

Roscoe_18
(membrane protein)

Roscoe_19
(major tail protein)

Roscoe_20

Roscoe_21
(queuine tRNA-ribosyltransferase)

Roscoe_22

Roscoe_23
(head-to-tail adaptor)

Roscoe_24

Roscoe_25

Roscoe_26
(tail assembly chaperone)

Roscoe_27

Roscoe_28

Roscoe_29
(tape measure protein)

Roscoe_30
(minor tail protein)

Roscoe_31
(minor tail protein)

Roscoe_32
(minor tail protein)

Roscoe_33
(minor tail protein)

Roscoe_34
(minor tail protein)

Roscoe_35

Roscoe_36

Roscoe_37

Roscoe_38

Roscoe_39

Roscoe_40

Roscoe_41

Roscoe_42

Roscoe_43

Roscoe_44

Roscoe_45
(membrane protein)

Roscoe_46
(helix-turn-helix DNA binding domain protein)

Roscoe_47
(helix-turn-helix DNA binding domain protein)

Roscoe_48

Roscoe_49
(lysin A)

Roscoe_50
(lysin B)

Roscoe_51

Roscoe_52

Roscoe_53
(DNA helicase)

Roscoe_54

Roscoe_55

Roscoe_56

Roscoe_57

Roscoe_58
(DNA primase/polymerase)

Roscoe_59

Roscoe_60

Roscoe_61
(DNA polymerase I)

Roscoe_62

Roscoe_63

Roscoe_64

Roscoe_65

Roscoe_66

Roscoe_67

Roscoe_68

Roscoe_69

Roscoe_70
(helix-turn-helix DNA binding domain protein)

Roscoe_71

Roscoe_72

Roscoe_73

Roscoe_74

Roscoe_75
(HNH endonuclease)

Roscoe_76

Roscoe_77

Roscoe_78

Roscoe_79

Roscoe_80

Roscoe_81

Roscoe_82

Roscoe_83

Roscoe_84

Roscoe_85
(HNH endonuclease)

Roscoe_86

Roscoe_87

Roscoe_88

Roscoe_89

Roscoe_90

Roscoe_91

Roscoe_92

Roscoe_93

Roscoe_94

Roscoe_95

Roscoe_96
(membrane protein)

Roscoe_97

Roscoe_98

Roscoe_99

Roscoe_100

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279877
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 9.9x10^10
Pitt Freezer Box# 5
Pitt Freezer Box Grid# B9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload