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Mycobacterium phage Weher20
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Detailed Information for Phage Weher20
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByS. Sobczyk, L. Cartagena, A. Papiez, J. Hamer
Year Found2020
Location FoundKenosha, WI United States
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates42.620259 N, 87.820516 W Map
Discovery NotesDiscovered in soil about 5.50 cm deep. It was about 22 degrees Celsius outside. SE of the Todd Weher building under three bushes. It was a thicker dry soil with wood chips, and you were able to see bush roots and decaying bush leaves on the soil. It was about a foot away from the first bush.
Naming NotesNamed after the building it found closest to paired with the year it was found.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage111
Genome length (bp)68684
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Dati (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

Prince (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Annotating InstitutionUniversity of the Ozarks
Annotation StatusIn GenBank
Plaque NotesThe phage plaque is about 1 mm in diameter with halos, which could indicate a temperate phage.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Weher20_1
(adenylate kinase)

Weher20_2
(terminase)

Weher20_3

Weher20_4

Weher20_5

Weher20_6
(RuvC-like resolvase)

Weher20_7

Weher20_8
(portal protein)

Weher20_9
(capsid maturation protease and MuF-like fusion protein)

Weher20_10
(HNH endonuclease)

Weher20_11

Weher20_12
(major capsid protein)

Weher20_13

Weher20_14

Weher20_15

Weher20_16

Weher20_17

Weher20_18
(major tail protein)

Weher20_19

Weher20_20
(queuine tRNA-ribosyltransferase)

Weher20_21

Weher20_22
(head-to-tail adaptor)

Weher20_23

Weher20_24

Weher20_25
(tail assembly chaperone)

Weher20_26

Weher20_27

Weher20_28
(tape measure protein)

Weher20_29
(minor tail protein)

Weher20_30
(minor tail protein)

Weher20_31
(minor tail protein)

Weher20_32
(minor tail protein)

Weher20_33
(minor tail protein)

Weher20_34

Weher20_35

Weher20_36

Weher20_37

Weher20_38

Weher20_39

Weher20_40

Weher20_41

Weher20_42

Weher20_43

Weher20_44

Weher20_45
(helix-turn-helix DNA binding domain)

Weher20_46
(helix-turn-helix DNA binding domain)

Weher20_47

Weher20_48
(lysin A)

Weher20_49
(lysin B)

Weher20_50

Weher20_51

Weher20_52
(DNA helicase)

Weher20_53

Weher20_54

Weher20_55

Weher20_56

Weher20_57

Weher20_58
(DNA primase/helicase)

Weher20_59

Weher20_60
(DNA polymerase I)

Weher20_61

Weher20_62

Weher20_63

Weher20_64

Weher20_65

Weher20_66

Weher20_67
(DNA binding protein)

Weher20_68

Weher20_69

Weher20_70

Weher20_71

Weher20_72

Weher20_73

Weher20_74

Weher20_75

Weher20_76

Weher20_77

Weher20_78

Weher20_79

Weher20_80

Weher20_81
(HNH endonuclease)

Weher20_82

Weher20_83

Weher20_84

Weher20_85

Weher20_86

Weher20_87

Weher20_88

Weher20_89

Weher20_90

Weher20_91

Weher20_92

Weher20_93

Weher20_94

Weher20_95

Weher20_96

Weher20_97

Weher20_98

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ681520
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 118
Pitt Freezer Box Grid# H1
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload