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Mycobacterium phage Ashraf
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Detailed Information for Phage Ashraf
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByImtiyaz Gulami (TATA CSIR OSDD Fellow)
Year Found2016
Location FoundMumbai, Maharashtra, India
Finding InstitutionAcharya Narendra Dev College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates19.228149 N, 72.845274 E Map
Discovery NotesIsolated from a soil sample procured from Ashraf Clinic, Sewri, Mumbai in a state called Maharashtra, India. The protocol followed for isolation of the Mycobacteriophages was as described in the manual "Phagehunting Teacher Workshop-2012". The work was carried out in Dr.Urmi Bajpai's laboratary at Department of Biomedical Science, Acharya Narendra Dev College, University of Delhi under PHAGE DISCOVERY Research Project (PDRP).
Naming NotesThe name of the phage is kept by the student Imtiyaz Gulami, TATA CSIR-OSDD Fellow who worked and discovered the Mycobacteriophage from the soil samples as a part of PHAGE DISCOVERY RESEARCH PROJECT (PDRP). The name ‘Ashraf’ stands for the location (Ashraf Clinic) from where the soil was obtained.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 3, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage957
Genome length (bp)69082
Character of genome endsCircularly Permuted
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Boehler (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionAcharya Narendra Dev College
Annotation StatusIn GenBank
Plaque NotesThe shape of the plaque which is circular ( 3 mm ) in diameter
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Ashraf_1
(adenylate kinase)

Ashraf_2
(terminase)

Ashraf_3

Ashraf_4

Ashraf_5

Ashraf_6
(RuvC-like resolvase)

Ashraf_7

Ashraf_8
(portal protein)

Ashraf_9
(capsid maturation protease and MuF-like fusion protein)

Ashraf_10

Ashraf_11
(major capsid subunit)

Ashraf_12

Ashraf_13

Ashraf_14

Ashraf_15

Ashraf_16

Ashraf_17
(major tail protein)

Ashraf_18

Ashraf_19
(queuine-tRNA ribosyltransferase)

Ashraf_20

Ashraf_21
(head-to-tail adaptor)

Ashraf_22

Ashraf_23

Ashraf_24
(tail assembly chaperone)

Ashraf_25

Ashraf_26

Ashraf_27
(tape measure protein)

Ashraf_28
(minor tail protein)

Ashraf_29
(minor tail protein)

Ashraf_30
(minor tail protein)

Ashraf_31
(minor tail protein)

Ashraf_32
(minor tail protein)

Ashraf_33

Ashraf_34

Ashraf_35

Ashraf_36

Ashraf_37

Ashraf_38

Ashraf_39

Ashraf_40

Ashraf_41

Ashraf_42

Ashraf_43

Ashraf_44
(helix-turn-helix DNA binding domain protein)

Ashraf_45
(HTH DNA binding protein)

Ashraf_46

Ashraf_47

Ashraf_48
(lysin A)

Ashraf_49
(lysin B)

Ashraf_50

Ashraf_51

Ashraf_52
(helicase)

Ashraf_53

Ashraf_54

Ashraf_55

Ashraf_56

Ashraf_57

Ashraf_58

Ashraf_59
(DNA primase/helicase)

Ashraf_60

Ashraf_61

Ashraf_62
(DNA polymerase I)

Ashraf_63

Ashraf_64
(RDF protein)

Ashraf_65

Ashraf_66

Ashraf_67

Ashraf_68

Ashraf_69

Ashraf_70
(putative HNH protein)

Ashraf_71
(RNaseE)

Ashraf_72

Ashraf_73

Ashraf_74
(PLA-2 like protein)

Ashraf_75

Ashraf_76
(HTH DNA binding domain protein)

Ashraf_77

Ashraf_78

Ashraf_79

Ashraf_80

Ashraf_81

Ashraf_82

Ashraf_83

Ashraf_84

Ashraf_85
(HNH endonuclease)

Ashraf_86

Ashraf_87

Ashraf_88

Ashraf_89

Ashraf_90

Ashraf_91

Ashraf_92

Ashraf_93

Ashraf_94

Ashraf_95

Ashraf_96

Ashraf_97

Ashraf_98

Ashraf_99

Ashraf_100

Ashraf_101

Ashraf_102

Ashraf_103

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKY385380
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload