Mycobacterium phage DirtJuice
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Detailed Information for Phage DirtJuice
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMatthew Scott, Ashley Terry, Jeniya Calloway
Year Found2019
Location FoundLodi, CA United States
Finding InstitutionVirginia Western Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates38.143853 N, 121.311323 W Map
Discovery NotesThe soil sample was collected in the back yard of a private residence in Lodi, California with GPS coordinates 38.143853, -121.311323. The soil is noted to have a dry moisture content by touch and light brown color. It has a fine sandy texture with mixed organic materials. The soil type is sandy loam. It was collected in a hot, dry area in full sun with the outside temperature approx. 104°F with low to no humidity
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 9, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage733
Genome length (bp)69166
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Bluephacebaby (B1)

Boehler (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Slatt (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Zenteno07 (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

LestyG (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionVirginia Western Community College
Annotation StatusIn GenBank
Plaque NotesMorphology on plates is noted to be larger and smaller plaques with halos noted around and clearing in the middle that ranges in sizes from 1-2 cm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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DirtJuice_1
(adenylate kinase)

DirtJuice_2
(terminase)

DirtJuice_3

DirtJuice_4

DirtJuice_5

DirtJuice_6
(RuvC-like resolvase)

DirtJuice_7

DirtJuice_8
(portal protein)

DirtJuice_9
(capsid maturation protease and MuF-like fusion protein)

DirtJuice_10
(HNH endonuclease)

DirtJuice_11

DirtJuice_12
(major capsid protein)

DirtJuice_13

DirtJuice_14

DirtJuice_15

DirtJuice_16

DirtJuice_17

DirtJuice_18
(major tail protein)

DirtJuice_19

DirtJuice_20
(queuine tRNA-ribosyltransferase)

DirtJuice_21

DirtJuice_22
(head-to-tail adaptor)

DirtJuice_23

DirtJuice_24

DirtJuice_25
(tail assembly chaperone)

DirtJuice_26

DirtJuice_27

DirtJuice_28
(tape measure protein)

DirtJuice_29
(minor tail protein)

DirtJuice_30
(minor tail protein)

DirtJuice_31
(minor tail protein)

DirtJuice_32
(minor tail protein)

DirtJuice_33
(minor tail protein)

DirtJuice_34

DirtJuice_35

DirtJuice_36

DirtJuice_37

DirtJuice_38

DirtJuice_39

DirtJuice_40

DirtJuice_41

DirtJuice_42

DirtJuice_43

DirtJuice_44

DirtJuice_45
(helix-turn-helix DNA binding domain protein)

DirtJuice_46
(helix-turn-helix DNA binding domain protein)

DirtJuice_47

DirtJuice_48
(lysin A)

DirtJuice_49
(lysin B)

DirtJuice_50

DirtJuice_51

DirtJuice_52
(DNA helicase)

DirtJuice_53

DirtJuice_54

DirtJuice_55

DirtJuice_56

DirtJuice_57

DirtJuice_58

DirtJuice_59
(DNA primase/polymerase)

DirtJuice_60

DirtJuice_61
(DNA polymerase I)

DirtJuice_62

DirtJuice_63

DirtJuice_64

DirtJuice_65

DirtJuice_66

DirtJuice_67

DirtJuice_68
(ribbon-helix-helix DNA binding domain protein)

DirtJuice_69

DirtJuice_70
(helix-turn-helix DNA binding domain protein)

DirtJuice_71

DirtJuice_72

DirtJuice_73

DirtJuice_74

DirtJuice_75

DirtJuice_76

DirtJuice_77

DirtJuice_78

DirtJuice_79

DirtJuice_80

DirtJuice_81

DirtJuice_82

DirtJuice_83

DirtJuice_84
(HNH endonuclease)

DirtJuice_85

DirtJuice_86

DirtJuice_87

DirtJuice_88

DirtJuice_89

DirtJuice_90

DirtJuice_91

DirtJuice_92

DirtJuice_93

DirtJuice_94

DirtJuice_95

DirtJuice_96

DirtJuice_97

DirtJuice_98

DirtJuice_99

DirtJuice_100

DirtJuice_101

DirtJuice_102

DirtJuice_103

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT897903
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 103
Pitt Freezer Box Grid# B2
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload