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Detailed Information for Phage DirtJuice
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Matthew Scott, Ashley Terry, Jeniya Calloway
Year Found	2019
Location Found	Lodi, CA United States
Finding Institution	Virginia Western Community College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	38.143853 N, 121.311323 W Map
Discovery Notes	The soil sample was collected in the back yard of a private residence in Lodi, California with GPS coordinates 38.143853, -121.311323. The soil is noted to have a dry moisture content by touch and light brown color. It has a fine sandy texture with mixed organic materials. The soil type is sandy loam. It was collected in a hot, dry area in full sun with the outside temperature approx. 104°F with low to no humidity
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 9, 2019
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	733
Genome length (bp)	69166
Character of genome ends	Circularly Permuted
GC Content	66.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Andre (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) Basato (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) BlueHusk (B1) Bluephacebaby (B1) Boehler (B1) Buckeye (B1) Burr (B1) CamL (B1) Cannibal (B1) Carthage (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Cher (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Cobra (B1) Colbert (B1) Cornobble (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) DaddyDaniels (B1) Daffy (B1) Daka (B1) Dati (B1) DelRivs (B1) Derpp (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Etaye (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) Fozzie (B1) Frankicide (B1) Freya (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giraffe (B1) Gophee (B1) Grand2040 (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Hartsy (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Inverness (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) Jillium (B1) Jiminy (B1) Kahve (B1) Kailash (B1) Katniss (B1) Keitherie (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) LasagnaCat (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) LeiMonet (B1) LemonSlice (B1) Longacauda (B1) Lopsy (B1) LostAndPhound (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Lumine (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megatron (B1) Melc17 (B1) MelsMeow (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) MiniBoss (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Nicole21 (B1) Numberten (B1) Nyala (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orion (B1) Orwigg (B1) OSmaximus (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhatLouie (B1) PhenghisKhan (B1) Pherdinand (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) RedMaple (B1) Riggan (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Schueller (B1) Scoot17C (B1) Scrick (B1) SDcharge11 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) Simielle (B1) Skippy (B1) Slatt (B1) Soile (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swiphy (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Timmi (B1) TomBombadil (B1) Tomlarah (B1) Toni (B1) Tooj (B1) True (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Usavi (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Virgeve (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Weher20 (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Hashim76 (B13) Zenteno07 (B13) Allegro (B2) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Calamitous (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kaleb (B2) Kheth (B2) Lars (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Phantasmagoria (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17a (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Bernardo (B3) Briakila (B3) Casbah (B3) ChaChing (B3) Chandler (B3) Compostia (B3) Corofin (B3) Daisy (B3) Deenasa (B3) Devonte (B3) Gadjet (B3) Gervas (B3) GlenHope (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Kronus (B3) LestyG (B3) Marley1013 (B3) MmasiCarm (B3) Mortcellus (B3) Morty007 (B3) Neos5 (B3) Nozo (B3) Obutu (B3) OrangeOswald (B3) Phaedrus (B3) Phayeta (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) SynergyX (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) AlanGrant (B4) Apex (B4) Austelle (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Hangman (B4) Heath (B4) Hydro (B4) JAMaL (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Mudslide (B4) Nanao (B4) Nigel (B4) Poster (B4) Prince (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) DigitDog (B5) Donny (B5) EastView9101 (B5) Mysterious (B5) Nangang (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) StinkyVeggy (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Funsized (B9) Knocker (B9) PenguinLover67 (B9) Quesadilla (B9)
Annotating Institution	Virginia Western Community College
Annotation Status	In GenBank
Plaque Notes	Morphology on plates is noted to be larger and smaller plaques with halos noted around and clearing in the middle that ranges in sizes from 1-2 cm
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View DirtJuice_1 (adenylate kinase) DirtJuice_2 (terminase) DirtJuice_3 DirtJuice_4 DirtJuice_5 DirtJuice_6 (RuvC-like resolvase) DirtJuice_7 DirtJuice_8 (portal protein) DirtJuice_9 (capsid maturation protease and MuF-like fusion protein) DirtJuice_10 (HNH endonuclease) DirtJuice_11 DirtJuice_12 (major capsid hexamer protein) DirtJuice_13 (major capsid pentamer protein) DirtJuice_14 DirtJuice_15 DirtJuice_16 DirtJuice_17 DirtJuice_18 (major tail protein) DirtJuice_19 DirtJuice_20 (queuine tRNA-ribosyltransferase) DirtJuice_21 DirtJuice_22 (head-to-tail adaptor) DirtJuice_23 DirtJuice_24 DirtJuice_25 (tail assembly chaperone) DirtJuice_26 DirtJuice_27 DirtJuice_28 (tape measure protein) DirtJuice_29 (minor tail protein) DirtJuice_30 (minor tail protein) DirtJuice_31 (minor tail protein) DirtJuice_32 (minor tail protein) DirtJuice_33 (minor tail protein) DirtJuice_34 DirtJuice_35 DirtJuice_36 DirtJuice_37 DirtJuice_38 DirtJuice_39 DirtJuice_40 DirtJuice_41 DirtJuice_42 DirtJuice_43 DirtJuice_44 DirtJuice_45 (helix-turn-helix DNA binding domain protein) DirtJuice_46 (helix-turn-helix DNA binding domain protein) DirtJuice_47 DirtJuice_48 (lysin A) DirtJuice_49 (lysin B) DirtJuice_50 DirtJuice_51 DirtJuice_52 (DNA helicase) DirtJuice_53 DirtJuice_54 DirtJuice_55 DirtJuice_56 DirtJuice_57 DirtJuice_58 DirtJuice_59 (DNA primase/polymerase) DirtJuice_60 DirtJuice_61 (DNA polymerase I) DirtJuice_62 DirtJuice_63 DirtJuice_64 DirtJuice_65 DirtJuice_66 DirtJuice_67 DirtJuice_68 (ribbon-helix-helix DNA binding domain protein) DirtJuice_69 DirtJuice_70 (helix-turn-helix DNA binding domain protein) DirtJuice_71 DirtJuice_72 DirtJuice_73 DirtJuice_74 DirtJuice_75 DirtJuice_76 DirtJuice_77 DirtJuice_78 DirtJuice_79 DirtJuice_80 DirtJuice_81 DirtJuice_82 DirtJuice_83 DirtJuice_84 (HNH endonuclease) DirtJuice_85 DirtJuice_86 DirtJuice_87 DirtJuice_88 DirtJuice_89 DirtJuice_90 DirtJuice_91 DirtJuice_92 DirtJuice_93 DirtJuice_94 DirtJuice_95 DirtJuice_96 DirtJuice_97 DirtJuice_98 DirtJuice_99 DirtJuice_100 DirtJuice_101 DirtJuice_102 DirtJuice_103
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MT897903
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	103
Pitt Freezer Box Grid#	B2
Available Files
Plaque Picture	Download
GenBank File for Phamerator	Download

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