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Mycobacterium phage Buckeye

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Detailed Information for Phage Buckeye
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJenna Nagy and Amanda Brehm
Year Found2014
Location FoundColumbus, OH USA
Finding InstitutionThe Ohio State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS CoordinatesUnavailable
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3297
Genome length (bp)69174
Character of genome endsCircularly Permuted
GC Content66.5%
Sequencing NotesDuring sequencing, it appeared that the sample contained two Cluster B1 phages that were fairly similar. This caused the assembler to break the genome into many pieces at the areas of disagreement. The genome was put back together based on coverage, since one "species" had about 3 times the coverage of the other, so all of the high coverage contigs were stitched together to make the Buckeye genome. Still, there was definitely a secondary genome in the DNA sample that was sequenced, and it was another Cluster B1 phage.
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Boehler (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Quesadilla (B9)

Annotating InstitutionThe Ohio State University
Annotation StatusIn GenBank
Plaque NotesLytic plaques, of 0.05cm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Buckeye_1
(adenylate kinase)

Buckeye_2
(terminase)

Buckeye_3

Buckeye_4

Buckeye_5

Buckeye_6
(RuvC-like resolvase)

Buckeye_7

Buckeye_8
(portal protein)

Buckeye_9
(capsid maturation protease and MuF-like fusion protein)

Buckeye_10
(HNH endonuclease)

Buckeye_11

Buckeye_12
(major capsid protein)

Buckeye_13

Buckeye_14

Buckeye_15

Buckeye_16

Buckeye_17

Buckeye_18
(major tail protein)

Buckeye_19

Buckeye_20
(queuine tRNA-ribosyltransferase)

Buckeye_21

Buckeye_22
(head-to-tail adaptor)

Buckeye_23

Buckeye_24

Buckeye_25
(tail assembly chaperone)

Buckeye_26

Buckeye_27

Buckeye_28
(tape measure protein)

Buckeye_29
(minor tail protein)

Buckeye_30
(minor tail protein)

Buckeye_31
(minor tail protein)

Buckeye_32

Buckeye_33

Buckeye_34

Buckeye_35

Buckeye_36

Buckeye_37

Buckeye_38

Buckeye_39

Buckeye_40

Buckeye_41

Buckeye_42

Buckeye_43

Buckeye_44

Buckeye_45
(helix-turn-helix DNA binding domain protein)

Buckeye_46
(helix-turn-helix DNA binding protein)

Buckeye_47

Buckeye_48
(lysin A)

Buckeye_49
(lysin B)

Buckeye_50

Buckeye_51

Buckeye_52
(DNA helicase)

Buckeye_53

Buckeye_54

Buckeye_55

Buckeye_56

Buckeye_57
(DNA primase/helicase)

Buckeye_58

Buckeye_59

Buckeye_60
(HNH endonuclease)

Buckeye_61
(DNA polymerase I)

Buckeye_62

Buckeye_63

Buckeye_64

Buckeye_65

Buckeye_66

Buckeye_67

Buckeye_68

Buckeye_69

Buckeye_70
(RNaseE)

Buckeye_71

Buckeye_72

Buckeye_73

Buckeye_74

Buckeye_75

Buckeye_76

Buckeye_77

Buckeye_78

Buckeye_79

Buckeye_80

Buckeye_81

Buckeye_82

Buckeye_83

Buckeye_84
(HNH endonuclease)

Buckeye_85

Buckeye_86

Buckeye_87

Buckeye_88

Buckeye_89

Buckeye_90

Buckeye_91

Buckeye_92

Buckeye_93

Buckeye_94

Buckeye_95

Buckeye_96

Buckeye_97

Buckeye_98

Buckeye_99

Buckeye_100

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH450116
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.1×10^14 pfu/mL
Pitt Freezer Box# 12
Pitt Freezer Box Grid# B3
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload