The Actinobacteriophage Database

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Detailed Information for Phage Vincenzo
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Gabrielle Gentile
Year Found	2014
Location Found	Pittsburgh, PA United States
Finding Institution	University of Pittsburgh
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	40.442252 N, 79.952284 W Map
Discovery Notes	I extracted my soil from a forest trail in Schenley Park on Wednesday, September 03, 2014 at 11:15 AM. The selected region was shaded by numerous trees. The soil was dark brown and moist and taken from the surface of of the ground. There was much plant growth surrounding the soil including grass and green plants. The weather conditions were warm, yet cool under the shade.
Naming Notes	I chose to name my phage Vincenzo because it is an unusual name to give a virus.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 6, 2015
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	194
Genome length (bp)	72139
Character of genome ends	Circularly Permuted
GC Content	68.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B4
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Alis (None) Azarath (None) BirdsNest (None) BlaqueIvy (None) Cally (None) Ceci11 (None) ChrIke (None) Conway (None) CptKIRK (None) DaHileesi (None) Indlovu (None) Kerrigan (None) LilMcDreamy (None) Loadofcarp (None) Mage (None) Meraxes (None) Muus (None) Olive00 (None) Pallavicini (None) RoseLilly (None) SeTi1 (None) Skye (None) Themis (None) TommyBoy (None) ABU (B1) Alex (B1) AltPhacts (B1) Altwerkus (B1) Antonia (B1) Apizium (B1) ArigunDD10 (B1) Ashraf (B1) AstrumP28 (B1) AyJay1824 (B1) Badfish (B1) Banjo (B1) BatteryCK (B1) Beaglebox (B1) BengaliBalla92 (B1) Beni05 (B1) Benjamin1706 (B1) Bishoperium (B1) BlackStallion (B1) BRod943 (B1) Buckeye (B1) BullsEye (B1) CamL (B1) Cannibal (B1) Carthage (B1) Ceraveeno (B1) Chah (B1) CharlieGBrown (B1) CheetO (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Cobra (B1) Coffean (B1) Colbert (B1) Cold1 (B1) Cosmolli16 (B1) Cowgirl (B1) Craff (B1) Crownjwl (B1) Daffy (B1) Derpp (B1) Dingo (B1) Dione (B1) Ditto (B1) Doc (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) DuffyC (B1) Durga (B1) Egahp (B1) Emiris (B1) EmmyMG (B1) EmpTee (B1) Eremos (B1) Eugenia (B1) Fang (B1) Ferhersher (B1) FluffyNinja (B1) Frankicide (B1) FriarPreacher (B1) Fringe (B1) Frogger (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giddykiddy (B1) Girly (B1) Gophee (B1) Grand2040 (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inkblot (B1) IPP (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) Jbone (B1) Jillium (B1) Josephine (B1) Kahve (B1) Kailash (B1) Kat63 (B1) Keitherie (B1) Kikipoo (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) Lasso (B1) Lauryn (B1) LeeLot (B1) Legion (B1) Lego3393 (B1) Legolas (B1) LemonSlice (B1) Lifestyle (B1) Llorona (B1) Longacauda (B1) LoveandHonor (B1) LuckyMarjie (B1) Lulumae (B1) Mag7 (B1) Maleldil (B1) Malkin71 (B1) Mana (B1) Manad (B1) Maskar (B1) Matalotodo (B1) Mecca (B1) Megatron (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) Mistert4 (B1) MitKao (B1) Morbach (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Novak (B1) Numberten (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orion (B1) OSmaximus (B1) Pacos19 (B1) PDRPv (B1) Pearsonia (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhenghisKhan (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Pickrel13 (B1) Piglet (B1) PinheadLarry (B1) PinkLady16 (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prann (B1) ProfessorX (B1) Puhltonio (B1) PuraVida (B1) Pyro (B1) QueenBeane (B1) RedMaple (B1) Riggan (B1) Robyn (B1) Rolex (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Scoot17C (B1) Scooterbug (B1) SDcharge11 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Speckles (B1) Squid (B1) Squiggle (B1) Squirt12 (B1) Steve27 (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swish (B1) TallGrassMM (B1) Thora (B1) ThreeOh3D2 (B1) Tigris (B1) Timmi (B1) TomBombadil (B1) Tre (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) Ulysses2016 (B1) UncleHowie (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Vista (B1) Vivaldi (B1) Vivamus (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Wunderlich (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Ypsitucky (B1) Zaider (B1) Zelda (B1) Zonia (B1) Arbiter (B2) Ares (B2) Boyle (B2) Brownie5 (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Julius (B2) Kheth (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) Opia (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17a (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Bernardo (B3) ChaChing (B3) Chandler (B3) CharlesTrout (B3) Compostia (B3) Corofin (B3) Daisy (B3) Gadjet (B3) Giraffey (B3) Gulsi (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Littleengine (B3) Marley1013 (B3) Mortcellus (B3) Morty007 (B3) Nozo (B3) OrangeOswald (B3) Phaedrus (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) RomaT (B3) SassBadger (B3) Tydolla (B3) Yahalom (B3) Zollars18 (B3) Adawi (B4) AlanGrant (B4) Bane1 (B4) Bane2 (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Halloween (B4) Hangman (B4) JAMaL (B4) LeapingLizzy (B4) Nigel (B4) Paliothales9 (B4) RawrgerThat (B4) Skruzzles (B4) Stinger (B4) TheRipper (B4) Tvorc (B4) Vincenzo (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) Donny (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Larry28 (B7) Saguaro (B7) Tapir (B7) Thonko (B8)
Annotation Status	In GenBank
Plaque Notes	A 10^4 dilution of phage Vincenzo was used to infect Mycobacterium smegmatis and plated on 7H10 medium. Plates were incubated at 30ºC for 48 hours. Vincenzo typically forms clear plaques with an average size <1mm, but some variation in plaque size (up to 1mm) is frequently observed.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Vincenzo_1 Vincenzo_2 (carboxylate deaminase) Vincenzo_3 Vincenzo_4 (terminase small subunit) Vincenzo_5 (terminase large subunit) Vincenzo_6 Vincenzo_7 Vincenzo_8 (RuvC) Vincenzo_9 Vincenzo_10 (portal protein) Vincenzo_11 (capsid morphogenesis protein) Vincenzo_12 Vincenzo_13 (major capsid protein) Vincenzo_14 Vincenzo_15 Vincenzo_16 Vincenzo_17 Vincenzo_18 Vincenzo_19 (major tail subunit) Vincenzo_20 Vincenzo_21 Vincenzo_22 Vincenzo_23 Vincenzo_24 Vincenzo_25 Vincenzo_26 (tapemeasure protein) Vincenzo_27 (minor tail protein) Vincenzo_28 (minor tail protein) Vincenzo_29 (minor tail protein) Vincenzo_30 (minor tail protein) Vincenzo_31 (minor tail protein) Vincenzo_32 Vincenzo_33 Vincenzo_34 Vincenzo_35 Vincenzo_36 Vincenzo_37 Vincenzo_38 Vincenzo_39 Vincenzo_40 Vincenzo_41 (AlpA-like protein) Vincenzo_42 (HTH DNA binding protein) Vincenzo_43 Vincenzo_44 (lysin A) Vincenzo_45 (lysin B) Vincenzo_46 Vincenzo_47 Vincenzo_48 Vincenzo_49 (DNA helicase) Vincenzo_50 Vincenzo_51 Vincenzo_52 Vincenzo_53 (primase/helicase) Vincenzo_54 Vincenzo_55 Vincenzo_56 (DNA polymerase I) Vincenzo_57 Vincenzo_58 Vincenzo_59 Vincenzo_60 Vincenzo_61 Vincenzo_62 Vincenzo_63 Vincenzo_64 Vincenzo_65 Vincenzo_66 Vincenzo_67 Vincenzo_68 Vincenzo_69 Vincenzo_70 (RNase E) Vincenzo_71 Vincenzo_72 Vincenzo_73 Vincenzo_74 Vincenzo_75 Vincenzo_76 Vincenzo_77 Vincenzo_78 (HNH endonuclease) Vincenzo_79 Vincenzo_80 Vincenzo_81 Vincenzo_82 Vincenzo_83 Vincenzo_84 Vincenzo_85 Vincenzo_86 Vincenzo_87 Vincenzo_88 Vincenzo_89 Vincenzo_90 Vincenzo_91 Vincenzo_92 Vincenzo_93 Vincenzo_94 Vincenzo_95 Vincenzo_96 Vincenzo_97 Vincenzo_98
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KR080194
Refseq Number	None yet
Published in Paper	Pope WH et al 2015, Genome Announcements: Genome sequence of Mycobacteriophage Momo
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	2
Pitt Freezer Box Grid#	B6
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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