| Detailed Information for Phage Vincenzo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gabrielle Gentile |
| Year Found | 2014 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.442252 N, 79.952284 W Map |
| Discovery Notes | I extracted my soil from a forest trail in Schenley Park on Wednesday, September 03, 2014 at 11:15 AM. The selected region was shaded by numerous trees. The soil was dark brown and moist and taken from the surface of of the ground. There was much plant growth surrounding the soil including grass and green plants. The weather conditions were warm, yet cool under the shade. |
| Naming Notes | I chose to name my phage Vincenzo because it is an unusual name to give a virus. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 194 |
| Genome length (bp) | 72139 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotation Status | In GenBank |
| Plaque Notes | A 10^4 dilution of phage Vincenzo was used to infect Mycobacterium smegmatis and plated on 7H10 medium. Plates were incubated at 30ºC for 48 hours. Vincenzo typically forms clear plaques with an average size <1mm, but some variation in plaque size (up to 1mm) is frequently observed. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR080194 |
| Refseq Number | None yet |
| Published in Paper | Pope WH et al 2015, Genome Announcements: Genome sequence of Mycobacteriophage Momo |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
B6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
