Mycobacterium phage Phamished
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Detailed Information for Phage Phamished
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesFrankenfeyge
Found BySarah Brantley
Year Found2014
Location FoundGettysburg, PA USA
Finding InstitutionGettysburg College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates39.834167 N, 77.234167 W Map
Discovery NotesFound outside of Schmucker Hall, the fine arts building at Gettysburg college.
Naming NotesThis phage literally devoured all of the M. smeg. My web plate was my -6 plate, and I had to go out to -11 to get my titer before the EM scan.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage205
Genome length (bp)68515
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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BirdsNest (None)

Indlovu (None)

LilMcDreamy (None)

Quesadilla (None)

AbsoluteMadLad (B1)

ABU (B1)

AltPhacts (B1)

Altwerkus (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

Boehler (B1)

Buckeye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Colbert (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jillium (B1)

Jiminy (B1)

Kahve (B1)

Kailash (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Skippy (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Timmi (B1)

TomBombadil (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

AlanGrant (B4)

Apex (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Hangman (B4)

JAMaL (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Nigel (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

Imvubu (B9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes101
Has been Phamerated?Yes
Gene List

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Phamished_1
(adenylate kinase)

Phamished_2
(terminase)

Phamished_3

Phamished_4

Phamished_5

Phamished_6
(RuvC-like resolvase)

Phamished_7

Phamished_8
(portal protein)

Phamished_9
(capsid maturation protease and MuF-like fusion protein)

Phamished_10
(HNH endonuclease)

Phamished_11

Phamished_12
(major capsid protein)

Phamished_13

Phamished_14

Phamished_15

Phamished_16

Phamished_17

Phamished_18
(major tail protein)

Phamished_19

Phamished_20
(queuine tRNA-ribosyltransferase)

Phamished_21

Phamished_22
(head-to-tail adaptor)

Phamished_23

Phamished_24

Phamished_25
(tail assembly chaperone)

Phamished_26

Phamished_27

Phamished_28
(tape measure protein)

Phamished_29
(minor tail subunit)

Phamished_30
(minor tail subunit)

Phamished_31
(minor tail subunit)

Phamished_32
(minor tail subunit)

Phamished_33
(minor tail subunit)

Phamished_34

Phamished_35

Phamished_36

Phamished_37

Phamished_38

Phamished_39

Phamished_40

Phamished_41

Phamished_42

Phamished_43

Phamished_44

Phamished_45
(helix-turn-helix DNA binding domain protein)

Phamished_46
(helix-turn-helix XRE-family like protein)

Phamished_47

Phamished_48

Phamished_49
(lysin A)

Phamished_50
(lysin B)

Phamished_51

Phamished_52

Phamished_53
(helicase type III subunit)

Phamished_54

Phamished_55

Phamished_56

Phamished_57

Phamished_58

Phamished_59
(DNA replicative helicase)

Phamished_60

Phamished_61

Phamished_62
(DNA polymerase I)

Phamished_63

Phamished_64

Phamished_65

Phamished_66

Phamished_67

Phamished_68

Phamished_69
(DNA binding domain protein)

Phamished_70

Phamished_71
(RNasE)

Phamished_72

Phamished_73

Phamished_74

Phamished_75
(DNA binding domain protein)

Phamished_76

Phamished_77

Phamished_78

Phamished_79

Phamished_80

Phamished_81

Phamished_82

Phamished_83

Phamished_84
(HNH endonuclease)

Phamished_85

Phamished_86

Phamished_87

Phamished_88

Phamished_89

Phamished_90

Phamished_91

Phamished_92

Phamished_93

Phamished_94

Phamished_95

Phamished_96
(TolA)

Phamished_97

Phamished_98

Phamished_99

Phamished_100

Phamished_101

Submitted Annotation in DNA Master FormatDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKR816508
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 15
Pitt Freezer Box Grid# C1
Available Files
Plaque PictureDownload
EM PictureDownload