| Detailed Information for Phage Allegro |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | CourtWalk |
| Found By | Ashley Wood, Courtney Miller,Gabby W, Mason Linker |
| Year Found | 2020 |
| Location Found | Rock Hill, SC United States |
| Finding Institution | Winthrop University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.9375 N, 81.033889 W Map |
| Discovery Notes | This phage was adopted from a classmate's enriched sample. The soil sample was collected one day after it rained. The outside temperature was cool, and the sample was collected about 2 cm deep. |
| Naming Notes | Allegro is a music term that means fast. This phage is very aggressive. Also, with this phage, we have worked very fast and efficient. This also fits with the name Allegro. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1957 |
| Genome length (bp) | 67439 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The phage is lytic. |
| Other Cluster Members |
|
| Annotating Institution | Winthrop University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque was tiny, about the size of a pen tip, and was clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ456036 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.67 x 10^9 pfu/mL |
| Pitt Freezer Box# |
118 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
