| Detailed Information for Phage Altwerkus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Augusto Ramirez |
| Year Found | 2013 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.331256 N, 76.620347 W Map |
| Discovery Notes | Soil Sample: (09/03/13 at 8:44 PM)
-Found in the John Hopkins University Bufano Gardens (North of the Undergraduate Teaching Lab)
-Air Temperature: 25°C
-Depth: 1.5 cm
-Moisture Content: Dry
-Soil was close to a tree and fallen leaves
-Insects crawling in the area
Phage Characteristics:
-Head Length: ~88 nm
-Head Width: ~83 nm
-Tail Length: ~418 nm |
| Naming Notes | Al(twerk)us. From the latin word altus, for tall, because of the great length of this phage's tail and the rising trend of this current generation called "twerking," which involves the movement of the tail. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 3, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 547 |
| Genome length (bp) | 68179 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | -Plaque Diameter: 2 mm - 3 mm
-Inside: Clear Plaques
-Shape: Very Circular and Rounded |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK112527 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
13 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
