| Detailed Information for Phage AngelCake |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Milani Parikh |
| Year Found | 2024 |
| Location Found | Merion Station, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.996221 N, 75.240382 W Map |
| Discovery Notes | In a dry mulch bed near a metal fence and some small shrubs. Close to a large tree. On a college campus close to dorm buildings. Very minimal foot traffic in that direct area. |
| Naming Notes | Our plaques have a halo surrounding the plaque that reminded us of an angel halo. The cake part was just for fun because we both love cake. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2525 |
| Genome length (bp) | 68698 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Small, clear, and circular plaques about 2mm in size. Surrounded by a turbid halo. The size of the halo extends up to 0.5 mm. All plates were incubated for 48 hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915842 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
212 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
