| Detailed Information for Phage Austelle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Autumn Vance and Estelle Arseneau |
| Year Found | 2021 |
| Location Found | North Andover, MA |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 66°C |
| GPS Coordinates | 42.67 N, 71.1213 W Map |
| Discovery Notes | Location: Outside of the Palmisano entrance, to the left of the Moseley Wrought
Bridge, next to the mulch bed.
Soil Quantity: 15mL in a conical tube
Temperature: 66° Fahrenheit, cloudy |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 877 |
| Genome length (bp) | 71799 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Merrimack College |
| Annotation Status | In GenBank |
| Plaque Notes | Our phage was temperate, this is
when the virus injects its DNA
into the bacteria but stays
dormant, you can tell this
because it looks “foggy” and not
clear. The plaque diameter was
small. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970575 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
137 |
| Pitt Freezer Box Grid# |
B6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
