| Detailed Information for Phage Baee |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Megan Ulbrich |
| Year Found | 2014 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.4417 N, 79.9512 W Map |
| Discovery Notes | Baee was found in the small forest area behind the Frick Fine Arts Building on the University of Pittsburgh campus. The soil was slightly dry, dark brown in color, and shaded by many trees at the time of extraction. Weather conditions were warm and the soil was collected about an inch from the surface of the area. |
| Naming Notes | Baee was named after the phrase "bae" which is the title that one may give their significant other rather than pet-names like "babe" or "baby." For example, one may say, "it's so nice to see you today, bae." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 13, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1007 |
| Genome length (bp) | 70270 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B5 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Phage Baee creates plaques on a Mycobacterium smegmatis lawn that are overall <1mm in size, circular in shape, and clear in appearance. Baee was subjected to 8 rounds of purifications preformed by infecting Mycobacterium smegmatis with Baee particles diluted 100-fold and plating on 7H10 agar prior to a 30oC incubation for 48 hours.
|
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR080199 |
| Refseq Number | None yet |
| Published in Paper | Pope WH et al 2015, Genome Announcements: Genome sequence of Mycobacteriophage Momo |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.3x10^9 pfu/ml |
| Date of SEA Lysate Titering |
Oct 19, 2014 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
H9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
