| Detailed Information for Phage Basato |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Justin A. Richardson |
| Year Found | 2021 |
| Location Found | Henderson, NV United States |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 36°C |
| GPS Coordinates | 35.9 N, 115.1 W Map |
| Discovery Notes | The solid soil sample was collected on 9/6/21 at 8:37PM from a residential planter in Henderson, NV, 89052. The planter was growing both tomato and basil, and has been kept in a predominantly shaded area. The soil had a silty composition and was very dark in color. It was moist soil with pieces of bark throughout. The sample was collected from approximately 5cm deep and had a wood-like scent. |
| Naming Notes | Named Basato due to the collection of soil coming from a planter growing tomato and basil. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 439 |
| Genome length (bp) | 68518 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | In GenBank |
| Plaque Notes | This phage exhibited two differing morphologies. The more prominent morphology was roughly 0.2 to 0.4cm in diameter, with turbid borders consistent with a more lysogenic phage. The other morphology present was 0.04 to 0.08cm in diameter, with more clear borders, but still characteristic of a temperate phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR159661 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.12 x 10^10 |
| Pitt Freezer Box# |
135 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
