| Detailed Information for Phage Bernardo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | C. Prien, N. Morales, A. Cohen, and R. Avetisyan |
| Year Found | 2012 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.074363 N, 118.44384 W Map |
| Discovery Notes | Isolated in MIMG 103AL (Spring 2012) and annotated in MIMG 103BL (Fall 2012) by Team RANC: Christopher Prien, Nicholis Morales, Alexa Cohen, and Rozeta Avetisyan
Instructor: Erin Sanders
TA (AL): Shannon Beaty
TA (BL): Will Villella
Curation of annotation results done by 199 students (Winter 2013): Shannon Cooper |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 22, 2013 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 28 |
| Genome length (bp) | 68196 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Turbid plaques suggest that the phage is lysogenic. However, the lack of key lysogenic pathway genes, such as integrases, is a cause for concern. The presence of an exonuclease and endonuclease indicates that the phage could have a lytic life cycle. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are turbid and homogenous with a diameter of 2mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF493879 |
| Refseq Number | NC_022983 |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012UCLAbernardoNM |
| SEA Lysate Titer |
4.6x10^10 |
| Date of SEA Lysate Titering |
May 24, 2012 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
D9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
