| Detailed Information for Phage BobbyK |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Deborah Jacobs-Sera |
| Year Found | 2023 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.560634 N, 80.010128 W Map |
| Discovery Notes | Phage was isolated from 50x concentrated wastewater collected from MTSA-Longview-1-STP on 10/5/23. Samples were concentrated on 10/13/23 after filtering (.22um) using 30kDa Centricon® Plus-70 Filter Units by Jakobi D, and tested for phage activity by Nate W. & Sergei E.-D. |
| Naming Notes | This phage name is a two-fer. It is named in honor of my brother-in-law, Bobby K. who passed away November 6, 2022. It is also named after my favorite nephew, Bobby K. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 473 |
| Genome length (bp) | 70887 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP750967 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| GenBank File for Phamerator | Download |