| Detailed Information for Phage Boehler |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Victoria Parker |
| Year Found | 2018 |
| Location Found | Saginaw, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 43.381475 N, 84.016327 W Map |
| Discovery Notes | Sample soil was gathered in a compost in the back of a greenhouse. It had rained the previous day so the soil was moist approximately 4-5 inches under the surface where I collected the sample. Soil was collected from compost mainly of rotten vegetables, fertilizer and dead plants making this an ideal candidate for phage samples for isolation. |
| Naming Notes | The name "Boehler" comes from the location in which I collected the sample from. Boehler's Greenhouse is a local, family owned business in which they were generous enough to allow a sample to be taken from their plant compost pile in the back. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1036 |
| Genome length (bp) | 68341 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Lagos |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are consistantly medium in size and turbid throughout. Density-Independent. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT897902 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.133 x 10^15 |
| Pitt Freezer Box# |
78 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
