| Detailed Information for Phage Boyle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Katy Heslop and John Cole |
| Year Found | 2016 |
| Location Found | Greensburg, PA USA |
| Finding Institution | Seton Hill University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 40.309898 N, 79.557391 W Map |
| Discovery Notes | Boyle was found under the stairs outside of Havey Hall at Seton Hill University. The climate was a warm September day, but did not reflect on the soil since the soil was under the stairs. |
| Naming Notes | Boyle got his name in honor of the building he was purified in was named Joanne Woodyard Boyle Health Science Center. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 21, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1043 |
| Genome length (bp) | 67491 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Seton Hill University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology showed the sizes of clearing on the L-agar plate. The plate was completely clear, which means it is lytic phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH051249 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
33 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| GenBank File for Phamerator | Download |