| Detailed Information for Phage Burr |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | April Pivonka |
| Year Found | 2018 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.9944 N, 75.2405 W Map |
| Discovery Notes | Burr was found under a tree in between the science center and Sweeney Field on the campus of Saint Joseph's University on September 4th, 2018. The weather was very sunny as it was the third day of a heat wave. The humidity was 57% and the ambient temperature was 32 degrees C. In addition, the depth of the soil collected was 3 cm. |
| Naming Notes | This phage is named after the character Aaron Burr from the musical Hamilton. His song "Wait For It" is representative of the fact that it took many attempts for the phage to form plaques to complete a spot pick. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 21, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1480 |
| Genome length (bp) | 68721 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | The phage produced clear, medium sized plaques which had an average range of 1 to 3 mm in diameter. The plaques were round with undefined edges and formed about 24-48 hours after incubation at 37 degrees C. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW712720 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
D7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |