Detailed Information for Phage CampRoach |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Katelyn Derstine and Lauren Zile |
Year Found | 2023 |
Location Found | Philadelphia, PA United States |
Finding Institution | Saint Joseph's University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 39.99357 N, 75.239111 W Map |
Discovery Notes | Soil sample was very dry and only about 7 cm deep. Achieved on a sunny day in a urban area with lots of foot traffic. The humidity was relatively low at about 60% and the temperature was 25 C. |
Naming Notes | This came from the overall location. We achieved the soil sample from outside the building "Campion" |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 12, 2024 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 2134 |
Genome length (bp) | 68968 |
Character of genome ends | Circularly Permuted |
GC Content | 66.3% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | B |
Subcluster | B1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Saint Joseph's University |
Annotation Status | In GenBank |
Plaque Notes | Plaques were typically turbid and bull's eye. No clear plaques. Around 3 mm in diameter. Plaques typically emerged after 48 hours in incubation |
Morphotype | Siphoviridae |
Number of Genes | 102 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | PP978877 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
186 |
Pitt Freezer Box Grid# |
H4 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |