| Detailed Information for Phage CampRoach |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Katelyn Derstine and Lauren Zile |
| Year Found | 2023 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.99357 N, 75.239111 W Map |
| Discovery Notes | Soil sample was very dry and only about 7 cm deep. Achieved on a sunny day in a urban area with lots of foot traffic. The humidity was relatively low at about 60% and the temperature was 25 C. |
| Naming Notes | This came from the overall location. We achieved the soil sample from outside the building "Campion" |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2134 |
| Genome length (bp) | 68968 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were typically turbid and bull's eye. No clear plaques. Around 3 mm in diameter. Plaques typically emerged after 48 hours in incubation |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978877 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
186 |
| Pitt Freezer Box Grid# |
H4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
