| Detailed Information for Phage Carthage |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Oliver Massey |
| Year Found | 2018 |
| Location Found | Auckland, New Zealand |
| Finding Institution | Massey University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.745556 S, 174.700833 E Map |
| Discovery Notes | Was found in a compost bin from direct plating. Bin was located behind the Massey University Albany campus cafeteria. |
| Naming Notes | Carthage was chosen as I have and interest in history. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 3, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1461 |
| Genome length (bp) | 67952 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Massey University |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques of medium/small size.
Note;
Appears to mutate to have a smaller morphology. Picking an isolated small plaque yields both medium and small plaques. However, Carthage has been purified so I don't think this is two phages |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524512 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.7X10^10 |
| Pitt Freezer Box# |
72 |
| Pitt Freezer Box Grid# |
D5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
