| Detailed Information for Phage CharlieGBrown |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jenny Samiec and Jackie Etson |
| Year Found | 2014 |
| Location Found | Columbus, OH USA |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 25°C |
| GPS Coordinates | 40.0013 N, 83.0146 W Map |
| Discovery Notes | Phage isolated in a biology lab at The Ohio State University. Possibly a temperate phage with a mostly lytic lifestyle. EcoRI made one cut on the phage's DNA during the RE digest. |
| Naming Notes | We decided to name it Charliegbrown because Christmas is coming! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1868 |
| Genome length (bp) | 68604 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were small, round, about 2mm in diameter. Clear most of the time. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX576647 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.96 x 10^14 pfu/mL |
| Date of SEA Lysate Titering |
Oct 20, 2014 |
| Pitt Freezer Box# |
12 |
| Pitt Freezer Box Grid# |
B4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
