| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joanna Smith & Brian Lott |
| Year Found | 2013 |
| Location Found | Santa Cruz, CA USA |
| Finding Institution | University of California, Santa Cruz |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.991525 N, 122.058426 W Map |
| Discovery Notes | Found in a compost pile at the UCSC Center for Agroecology, about 1 inch deep in the compost pile. Phage discovered, purified, and named by Joanna Smith. DNA purification and restriction digests were performed by Brian Lott. |
| Naming Notes | Two separate phages were isolated from the same sample. During purification their morphologies were noticeably different, one was small (called 'Teenie') and the other was very large (appropriately named 'Chunky'.) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1941 |
| Genome length (bp) | 68249 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of California, Santa Cruz |
| Annotation Status | In GenBank |
| Plaque Notes | Chunky forms large, clear plaques that range in size from 3mm to 5mm. Average plaque size would be ~3.5mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG925339 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.5x10^9 pfu/mL |
| Date of SEA Lysate Titering |
Nov 27, 2013 |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
32 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
