| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexander J David Hallwachs |
| Year Found | 2012 |
| Location Found | Saint Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.644038 N, 90.314121 W Map |
| Discovery Notes | The environmental sample was collected from the mulched area around a sapling, about 4 centimeters deep at the roots of a fungi growing in the mulch. The soil was dry and had a slight southeast slope. The temperature was roughly 26.11 degrees Celsius (79 degrees Fahrenheit). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 23, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 165 |
| Genome length (bp) | 68015 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Cabrini University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques have a consistent morphology of a clear circular area of about 1 millimeter in diameter and a oval shaped turbid area around the clear area about 2 millimeters wide and 3 millimeters long. The clear area ranges in position in the turbid area but generally shifts lengthwise. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH576970 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012WUSLdonsanchonKH |
| SEA Lysate Titer |
4.3 x 10^9 |
| Date of SEA Lysate Titering |
Oct 4, 2012 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
