The Actinobacteriophage Database

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Detailed Information for Phage Durga
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Kasey Wagner
Year Found	2016
Location Found	Manhattan, KS USA
Finding Institution	Kansas State University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	22°C
GPS Coordinates	39.192363 N, 96.585999 W Map
Discovery Notes	Soil was collected on surface underneath a bush, behind a living complex. Soil was dry, with a few clumps, and mulch was present. The temperature was 22.2 degrees Celsius. Durga was collected August 24, 2016. Kasey Wagner discovered the phage, but Madeline Barger and Sydney Klein assisted in categorizing it.
Naming Notes	While completing multiple spot tests with the phage, the team noticed how aggressive Durga was. After researching names that would match our phage's intensity, Kasey Wagner stumbled upon the name Durga. This is the hindi goddess of war, who rides in on a lion or tiger, she has 9 arms all holding different weapons. As female scientists we connected with the ferocity or this name and saw it fit to give to our discovered phage.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 5, 2017
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	966
Genome length (bp)	68866
Character of genome ends	Circularly Permuted
GC Content	66.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Andre (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) Basato (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) BlueHusk (B1) Bluephacebaby (B1) Boehler (B1) Brilliant (B1) Buckeye (B1) Burr (B1) CamL (B1) CampRoach (B1) Cannibal (B1) Carthage (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Cher (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Chute (B1) Cobra (B1) Colbert (B1) Coletti (B1) Cornobble (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) DaddyDaniels (B1) Daffy (B1) Daka (B1) Dati (B1) DelRivs (B1) Derpp (B1) Dice (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Etaye (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) Fozzie (B1) Frankicide (B1) Freya (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giraffe (B1) Gophee (B1) Grand2040 (B1) Gwilliam (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Hartsy (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Inverness (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) JDog (B1) Jillium (B1) Jiminy (B1) Jiraiya (B1) Kahve (B1) Kailash (B1) Katniss (B1) Keitherie (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) LasagnaCat (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) LeiMonet (B1) LemonSlice (B1) Longacauda (B1) Lopsy (B1) LostAndPhound (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Lumine (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megamind20 (B1) Megatron (B1) Melc17 (B1) MelsMeow (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) MiniBoss (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) MrPhizzler (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Nicole21 (B1) Numberten (B1) Nyala (B1) Olak (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orfeu (B1) Orion (B1) Orwigg (B1) OSmaximus (B1) Pacifista (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhatLouie (B1) PhenghisKhan (B1) Pherdinand (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) Quisquiliae (B1) RedMaple (B1) Ricotta (B1) Riggan (B1) Rimu (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Schueller (B1) Scoot17C (B1) Scrick (B1) SDcharge11 (B1) Selr12 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) Simielle (B1) Skippy (B1) Slatt (B1) Soile (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swiphy (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Thunderbird (B1) Timmi (B1) TomBombadil (B1) Tomlarah (B1) Toni (B1) Tooj (B1) True (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Usavi (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Virgeve (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Weher20 (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Hashim76 (B13) Zenteno07 (B13) Allegro (B2) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Calamitous (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kaleb (B2) Kheth (B2) Lars (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Phantasmagoria (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17A (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Ben11 (B3) Bernardo (B3) Briakila (B3) Casbah (B3) ChaChing (B3) Chandler (B3) Compostia (B3) Corofin (B3) Daisy (B3) Deenasa (B3) Devonte (B3) Gadjet (B3) Gervas (B3) GlenHope (B3) Halfpint (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Kronus (B3) LestyG (B3) Marley1013 (B3) MmasiCarm (B3) Mortcellus (B3) Morty007 (B3) Neos5 (B3) Nozo (B3) Obutu (B3) OrangeOswald (B3) Phaedrus (B3) Phayeta (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) SlippinJimmy (B3) SynergyX (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) AlanGrant (B4) Apex (B4) Austelle (B4) BobbyK (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Hangman (B4) Heath (B4) Hydro (B4) JaguarMi (B4) JAMaL (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Mudslide (B4) Nanao (B4) Nigel (B4) Poster (B4) Prince (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) DigitDog (B5) Donny (B5) EastView9101 (B5) Mysterious (B5) Nangang (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) SirJeffery (B5) StinkyVeggy (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Funsized (B9) Knocker (B9) PenguinLover67 (B9) Quesadilla (B9)
Annotating Institution	Kansas State University
Annotation Status	In GenBank
Plaque Notes	In the Digest picture, only the first six lanes are of Durga.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Durga_1 (adenylate kinase) Durga_2 (terminase) Durga_3 Durga_4 Durga_5 Durga_6 (RuvC-like resolvase) Durga_7 Durga_8 (portal protein) Durga_9 (capsid maturation protease and MuF-like fusion protein) Durga_10 Durga_11 Durga_12 (major capsid hexamer protein) Durga_13 (major capsid pentamer protein) Durga_14 Durga_15 Durga_16 Durga_17 Durga_18 (major tail protein) Durga_19 Durga_20 (queuine tRNA-ribosyltransferase) Durga_21 Durga_22 (head-to-tail adaptor) Durga_23 Durga_24 Durga_25 (tail assembly chaperone) Durga_26 Durga_27 Durga_28 (tape measure protein) Durga_29 (minor tail protein) Durga_30 (minor tail protein) Durga_31 (minor tail protein) Durga_32 (minor tail protein) Durga_33 (minor tail protein) Durga_34 Durga_35 Durga_36 Durga_37 Durga_38 Durga_39 Durga_40 Durga_41 Durga_42 Durga_43 Durga_44 Durga_45 (helix-turn-helix DNA binding domain protein) Durga_46 (helix-turn-helix DNA binding domain protein) Durga_47 Durga_48 Durga_49 (lysin A) Durga_50 (lysin B) Durga_51 Durga_52 Durga_53 (DNA helicase) Durga_54 Durga_55 Durga_56 Durga_57 Durga_58 Durga_59 Durga_60 (DNA primase/helicase) Durga_61 Durga_62 Durga_63 (DNA polymerase I) Durga_64 Durga_65 Durga_66 Durga_67 Durga_68 Durga_69 Durga_70 Durga_71 Durga_72 Durga_73 Durga_74 Durga_75 Durga_76 Durga_77 Durga_78 Durga_79 Durga_80 Durga_81 Durga_82 Durga_83 Durga_84 Durga_85 Durga_86 (HNH endonuclease) Durga_87 Durga_88 Durga_89 Durga_90 Durga_91 Durga_92 Durga_93 Durga_94 Durga_95 Durga_96 Durga_97 Durga_98 Durga_99 Durga_100 Durga_101 Durga_102 Durga_103
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK279850
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Designator	Durga
SEA Lysate Titer	2.67 X 10^9
Date of SEA Lysate Titering	Oct 11, 2016
Pitt Freezer Box#	35
Pitt Freezer Box Grid#	A1
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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